| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSH2 |
| Uniprot No | Q76I76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1423aa |
| 氨基酸序列 | MALVTVQRSPTPSTTSSPCASEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTPRISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTMGLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWSALQSLHKACEVARAHNYYPGSLFLTWVSYYESHINSDQSSVNEWNAMQDVQSHRPDSPALFTDIPTERERTERLIKTKLREIMMQKDLENITSKEIRTELEMQMVCNLREFKEFIDNEMIVILGQMDSPTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREIDNFFPGVFEYHNIRVYDEEATDLLAYWNDTYKFISKAKKHGSKCLVHCKMGVSRSASTVIAYAMKEYGWNLDRAYDYVKERRTVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSDHHEPICKPGLELNKKDITTSADQIAEVKTMESHPPIPPVFVEHMVPQDANQKGLCTKERMICLEFTSREFHAGQIEDELNLNDINGCSSGCCLNESKFPLDNCHASKALIQPGHVPEMANKFPDLTVEDLETDALKADMNVHLLPMEELTSPLKDPPMSPDPESPSPQPSCQTEISDFSTDRIDFFSALEKFVELSQETRSRSFSHSRMEELGGGRNESCRLSVVEVAPSKVTADDQRSSSLSNTPHASEESSMDEEQSKAISELVSPDIFMQSHSENAISVKEIVTEIESISQGVGQIQLKGDILPNPCHTPKKNSIHELLLERAQTPENKPGHMEQDEDSCTAQPELAKDSGMCNPEGCLTTHSSIADLEEGEPAEGEQELQGSGMHPGAKWYPGSVRRATLEFEERLRQEQEHHGAAPTCTSLSTRKNSKNDSSVADLAPKGKSDEAPPEHSFVLKEPEMSKGKGKYSGSEAGSLSHSEQNATVPAPRVLEFDHLPDPQEGPGSDTGTQQEGVLKDLRTVIPYQESETQAVPLPLPKRVEIIEYTHIVTSPNHTGPGSEIATSEKSGEQGLRKVNMEKSVTVLCTLDENLNRTLDPNQVSLHPQVLPLPHSSSPEHNRPTDHPTSILSSPEDRGSSLSTALETAAPFVSHTTHLLSASLDYLHPQTMVHLEGFTEQSSTTDEPSAEQVSWEESQESPLSSGSEVPYKDSQLSSADLSLISKLGDNTGELQEKMDPLPVACRLPHSSSSENIKSLSHSPGVVKERAKEIESRVVFQAGLTKPSQMRRSASLAKLGYLDLCKDCLPEREPASCESPHLKLLQPFLRTDSGMHAMEDQESLENPGAPHNPEPTKSFVEQLTTTECIVQSKPVERPLVQYAKEFGSSQQYLLPRAGLELTSSEGGLPVLQTQGLQCACPAPGLAVAPRQQHGRTHPLRRLKKANDKKRTTNPFYNTM |
| 预测分子量 | 158,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SSH2重组蛋白的示例参考文献(内容为假设性示例，建议通过学术数据库查询真实文献)：
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1. **文献名称**: *Structural and Functional Analysis of Recombinant SSH2 Phosphatase*
**作者**: Niwa R, Tanaka K, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了SSH2重组蛋白，利用X射线晶体学解析了其三维结构，揭示了其与cofilin结合的活性位点，为调控细胞骨架动态性提供了结构基础。
2. **文献名称**: *SSH2-Mediated Cofilin Dephosphorylation in Cancer Cell Invasion*
**作者**: Nagata T, Yamaguchi H, et al.
**摘要**: 通过体外重组SSH2蛋白实验，证明其通过去磷酸化cofilin促进肿瘤细胞伪足形成和迁移，提示SSH2可能成为抑制癌症转移的潜在靶点。
3. **文献名称**: *Development of a High-Throughput Screen for SSH2 Inhibitors Using Recombinant Protein*
**作者**: Lee S, Kim J, et al.
**摘要**: 基于重组SSH2蛋白建立了药物筛选平台，鉴定出小分子抑制剂X-123.可特异性阻断SSH2-cofilin通路，为抗纤维化治疗提供新策略。
4. **文献名称**: *SSH2 Recombinant Protein Regulates Neuronal Axon Guidance*
**作者**: Huang L, Zhang W, et al.
**摘要**: 利用重组SSH2蛋白探究其在神经元发育中的作用，发现其通过调控肌动蛋白重组参与轴突导向，为神经再生研究提供新方向。
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建议通过 **PubMed/Google Scholar** 检索关键词 `SSH2 recombinant protein` 或 `Slingshot phosphatase 2` 获取真实文献。
**Background of SSH2 Recombinant Protein**
The SSH2 (Slingshot Homolog 2) protein is a member of the Slingshot phosphatase family, which plays a critical role in regulating actin cytoskeleton dynamics by dephosphorylating and reactivating cofilin, an essential actin-binding protein. Cofilin, when active, severs and depolymerizes actin filaments, enabling cytoskeletal remodeling—a process vital for cell motility, division, and signaling. SSH2 specifically counteracts LIM kinase (LIMK)-mediated phosphorylation of cofilin, restoring its actin-disassembling activity. This regulatory mechanism is crucial in diverse physiological processes, including neuronal development, immune cell migration, and cancer metastasis.
SSH2 contains conserved phosphatase domains and regulatory regions that interact with actin-binding proteins, ensuring spatiotemporal control of cofilin activation. Dysregulation of SSH2-cofilin signaling is implicated in pathological conditions, such as tumor invasion, neurodegenerative disorders, and inflammatory diseases. For instance, reduced SSH2 activity in cancer cells can lead to hyperphosphorylated cofilin, stabilizing actin filaments and promoting invasive behavior.
Recombinant SSH2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables detailed study of its structure-function relationships, enzymatic kinetics, and interaction partners. It serves as a tool for screening modulators of cofilin phosphorylation, with therapeutic potential in diseases linked to cytoskeletal dysfunction. Recent studies also explore engineered SSH2 variants to enhance stability or substrate specificity, advancing its application in biomedical research and drug development.
In summary, SSH2 recombinant protein provides a molecular lens to dissect actin dynamics regulation, offering insights into both basic cell biology and translational medicine. Its study continues to uncover novel targets for therapies aimed at cytoskeleton-related pathologies. (Word count: 298)
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