| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SSH1 |
| Uniprot No | Q8WYL5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1049aa |
| 氨基酸序列 | MALVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFMVKGAALFLQQGSSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDFLPETPDGTPESQLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAEVHRPARQPQQGSGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLNNNSKRSCPNGMEDDAIFGILNKVKPSYKSCADCMYPTASGAPEASRERCEDPNAPAICTQPAFLPHITSSPVAHLASRSRVPEKPASGPTEPPPFLPPAGSRRADTSGPGAGAALEPPASLLEPSRETPKVLPKSLLLKNSHCDKNPPSTEVVIKEESSPKKDMKPAKDLRLLFSNESEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSVPADPAPPSRDGPASRLEASIPEESQDPAALHELGPLVMPSQAGSDEKSEAAPASLEGGSLKSPPPFFYRLDHTSSFSKDFLKTICYTPTSSSMSSNLTRSSSSDSIHSVRGKPGLVKQRTQEIETRLRLAGLTVSSPLKRSHSLAKLGSLTFSTEDLSSEADPSTVADSQDTTLSESSFLHEPQGTPRDPAATSKPSGKPAPENLKSPSWMSKS |
| 预测分子量 | 115,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SSH1重组蛋白的虚构参考文献示例(仅供格式参考，非真实文献)：
1. **文献名称**：*Expression and Purification of Recombinant SSH1 Phosphatase in Escherichia coli*
**作者**：Li, X., et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效制备SSH1重组蛋白的方法，优化了纯化条件并验证了其磷酸酶活性，为体外研究SSH1调控cofilin去磷酸化的机制提供了工具。
2. **文献名称**：*Structural Insights into SSH1-Mediated Cofilin Activation in Actin Remodeling*
**作者**：Wang, Y., & Zhang, H.
**摘要**：利用重组SSH1蛋白进行结晶和结构分析，揭示了SSH1与cofilin结合的特定结构域，阐明了其磷酸酶活性调控肌动蛋白丝动态组装的分子基础。
3. **文献名称**：*SSH1 Recombinant Protein Attenuates Neuronal Degeneration in Alzheimer’s Disease Models*
**作者**：Chen, J., et al.
**摘要**：在阿尔茨海默病细胞模型中，外源性添加SSH1重组蛋白可增强cofilin活性，改善突触功能，提示其潜在治疗神经退行性疾病的可行性。
4. **文献名称**：*Regulation of SSH1 Phosphatase Activity by LIM Kinase in Cancer Cell Migration*
**作者**：Kim, S., et al.
**摘要**：通过重组SSH1蛋白与LIM激酶的体外互作实验，证实LIM激酶通过磷酸化负调控SSH1活性，从而影响肿瘤细胞的迁移和侵袭能力。
注：以上内容为模拟文献，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
SSH1 (Slingshot Homolog 1) is a phosphatase that plays a critical role in regulating actin cytoskeleton dynamics, a fundamental process in cell motility, morphogenesis, and signaling. It belongs to the Slingshot family of proteins, initially identified in Drosophila for their role in photoreceptor development. SSH1 specifically dephosphorylates and reactivates cofilin, an actin-severing protein essential for actin filament disassembly. Cofilin activity is tightly controlled by phosphorylation (inactivation) via LIM kinases and dephosphorylation (activation) via SSH1. enabling precise spatiotemporal regulation of actin remodeling.
Inactive cofilin is phosphorylated at Ser-3. and SSH1-mediated dephosphorylation restores its ability to bind and sever actin filaments, promoting cytoskeletal reorganization. This dynamic process is vital for cellular functions such as migration, cytokinesis, and intracellular trafficking. Dysregulation of SSH1-cofilin signaling is implicated in pathological conditions, including cancer metastasis, neurodegenerative diseases, and immune cell dysfunction.
Recombinant SSH1 protein, produced through molecular cloning and expression systems (e.g., E. coli or mammalian cells), retains enzymatic activity and is widely used to study actin dynamics in vitro. Researchers employ it to dissect SSH1’s interaction with cofilin, its regulatory mechanisms (e.g., binding to actin or scaffolding proteins), and responses to upstream signals like Rho GTPases or calcium fluxes. Additionally, recombinant SSH1 serves as a tool for high-throughput drug screening to identify modulators of actin remodeling, with therapeutic potential in diseases involving aberrant cell motility or cytoskeletal defects. Its study enhances understanding of cell mechanics and offers pathways for targeted interventions in cancer and neurological disorders.
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