| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GYPE |
| Uniprot No | P15421 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-78aa |
| 氨基酸序列 | MYGKIIFVLLLSGIVSISASSTTGVAMHTSTSSSVTKSYISSQTNGITLINWWAMARVIFEVMLVVVGMIILISYCIR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于红细胞膜蛋白相关研究的推测性参考文献示例(请注意并非真实文献，仅供参考)：
1. **《Glycophorin E recombinant protein as a malaria receptor: Structural and functional analysis》**
- 作者：Springer, G.F. et al.
- 摘要：研究通过重组技术表达GYPE蛋白，解析其结构与恶性疟原虫入侵红细胞的相互作用，揭示GYPE作为潜在疟疾受体的关键区域。
2. **《Recombinant GYPE expression in HEK293 cells: Implications for blood group antigen studies》**
- 作者：Lomas-Francis, C. & Anstee, D.J.
- 摘要：构建GYPE重组蛋白表达系统，分析其携带的稀有血型抗原表位，为输血医学中的血型鉴定提供新工具。
3. **《Role of glycophorin E in red cell membrane integrity: Insights from recombinant protein models》**
- 作者：Mohandas, N. & Gallagher, P.G.
- 摘要：利用重组GYPE研究其在红细胞膜机械稳定性中的作用，发现GYPE缺失可能导致细胞形态异常及溶血风险增加。
4. **《Proteomic characterization of recombinant GYPE and its interaction with bacterial pathogens》**
- 作者：Tomita, M. & Marchesi, V.T.
- 摘要：通过质谱和免疫共沉淀技术，揭示重组GYPE与某些细菌表面蛋白的结合能力，提示其参与宿主先天免疫防御机制。
**注意**：以上为模拟文献，实际研究需通过PubMed/Google Scholar等平台以“GYPE recombinant protein”“Glycophorin E expression”等关键词检索。
**Background of GYPE Recombinant Protein**
The GYPE (Glycophorin E) gene encodes a minor sialoglycoprotein component of the erythrocyte membrane, belonging to the glycophorin family, which includes Glycophorin A (GYPA) and Glycophorin B (GYPB). These proteins are integral to red blood cell (RBC) structure and function, contributing to membrane stability, antigenic properties, and interactions with pathogens. GYPE shares significant homology with GYPB and is thought to have arisen through gene duplication events during evolution. However, unlike GYPA and GYPB, which carry the M/N and S/s blood group antigens, GYPE lacks well-characterized blood group activity and is expressed at very low levels on RBCs, making its biological role less understood.
Recombinant GYPE protein is engineered to study its structural and functional properties in vitro. Produced using expression systems like mammalian cells (e.g., CHO or HEK293) or *E. coli*, the recombinant protein typically retains key domains, such as the extracellular glycosylated region and transmembrane segment. This enables investigations into its potential roles in cell adhesion, pathogen binding (e.g., malaria parasite *Plasmodium falciparum*), or modulation of immune responses. GYPE has garnered interest due to its genetic linkage to GYPB and its possible involvement in hybrid glycophorin variants associated with blood group anomalies or disease susceptibility.
Research on GYPE recombinant protein also supports advancements in transfusion medicine, aiding in the characterization of rare blood group phenotypes and the development of diagnostic tools. Additionally, its study may shed light on evolutionary mechanisms within the glycophorin gene cluster and inform therapeutic strategies targeting RBC-pathogen interactions. Despite its low abundance, GYPE’s conserved sequence across primates suggests functional significance, driving ongoing exploration into its contributions to erythrocyte biology and disease.
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