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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDGFA |
| Uniprot No | P04085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 87-211aa |
| 氨基酸序列 | SIEE AVPAVCKTRT VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV KVAKVEYVRK KPKLKEVQVR LEEHLECACA TTSLNPDYRE EDTGRPRESG KKRKRKRLKP T |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDGFA重组蛋白的3篇参考文献，按研究方向和内容分类整理：
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### 1. **重组PDGFA的表达与功能验证**
**文献名称**：*"Production and Characterization of Recombinant Human PDGFA for Stem Cell Culture Applications"*
**作者**：Smith J. et al.
**摘要**：该研究通过大肠杆菌表达系统成功制备了高纯度重组人PDGFA蛋白，并验证其生物活性。实验表明，重组PDGFA可显著促进间充质干细胞的增殖和迁移，为体外细胞培养提供了关键生长因子支持。
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### 2. **PDGFA在肿瘤微环境中的作用**
**文献名称**：*"Recombinant PDGFA Enhances Angiogenesis in Glioblastoma Models by Activating PDGFRα Signaling"*
**作者**：Chen L. et al.
**摘要**：研究利用重组PDGFA蛋白处理胶质母细胞瘤小鼠模型，发现其通过激活PDGFRα受体，诱导肿瘤血管生成并加速肿瘤生长。结果表明，靶向PDGFA/PDGFRα轴可能成为癌症治疗的潜在策略。
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### 3. **结构解析与突变体功能分析**
**文献名称**：*"Crystal Structure of Recombinant PDGFA and Insights into Receptor Binding Specificity"*
**作者**：Wang Y. et al.
**摘要**：本研究解析了重组PDGFA蛋白的晶体结构，揭示了其与PDGFR结合的特定结构域。通过定点突变实验，发现某些氨基酸残基对受体激活至关重要，为设计PDGFA抑制剂提供了结构基础。
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**备注**：以上文献为示例，实际引用时请根据具体研究领域在PubMed或Web of Science中检索最新文章(可使用关键词：PDGFA recombinant protein, PDGFR signaling, angiogenesis, cancer therapy)。
**Background of PDGFA Recombinant Protein**
Platelet-derived growth factor alpha polypeptide (PDGFA) is a key member of the platelet-derived growth factor (PDGF) family, which includes dimeric ligands (PDGF-AA, BB, AB, CC, DD) involved in cellular proliferation, migration, and survival. PDGFA specifically binds to PDGF receptor-alpha (PDGFR-α), activating downstream signaling pathways such as MAPK/ERK and PI3K/AKT, which regulate embryogenesis, angiogenesis, and tissue repair.
Recombinant PDGFA is produced using genetic engineering techniques, often expressed in *E. coli*, yeast, or mammalian cell systems* to ensure high purity and bioactivity. The protein typically exists as a homodimer (PDGF-AA) with each monomer comprising approximately 16–18 kDa, depending on post-translational modifications like glycosylation. Its structure includes conserved cysteine residues critical for receptor-binding and dimer stability.
Research applications of PDGFA recombinant protein span *in vitro* cell culture studies (e.g., stimulating fibroblast proliferation, smooth muscle cell migration) and *in vivo* models to explore wound healing, vascular development, or pathological conditions like cancer and fibrosis. Overexpression of PDGFA is linked to tumor angiogenesis and stromal activation, making it a therapeutic target. Conversely, recombinant PDGFA has potential in regenerative medicine, such as enhancing tissue engineering scaffolds or treating chronic ulcers.
Quality control metrics for recombinant PDGFA include SDS-PAGE, Western blotting, and functional assays (e.g., receptor phosphorylation assays) to confirm consistency. Challenges in its use involve balancing its pro-growth effects with risks of promoting undesired cell proliferation or fibrosis. Ongoing studies aim to optimize delivery systems and dosage for clinical applications.
In summary, PDGFA recombinant protein is a versatile tool for studying developmental biology, disease mechanisms, and therapeutic strategies, underpinned by its central role in cell signaling and tissue homeostasis.
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