| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PFKP |
| Uniprot No | Q01813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-784aa |
| 氨基酸序列 | MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMG IYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQA FRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELAR NGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDA IMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEE QMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDT RVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGN HAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIK LPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGF DGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSI NALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSI GADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGA DAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFI YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAK LKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQW WLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV |
| 预测分子量 | 112 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PFKP(磷酸果糖激酶血小板型)重组蛋白的3篇参考文献示例(注：内容为模拟示例，非真实文献)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of Human Platelet-Type Phosphofructokinase (PFKP) in Escherichia coli"*
**作者**: Zhang Y, et al.
**摘要**: 该研究报道了利用大肠杆菌表达系统成功表达并纯化重组人源PFKP蛋白，通过酶动力学实验证实其催化果糖-6-磷酸转化为果糖-1.6-二磷酸的活性，并探讨了ATP/AMP浓度对其活性的调节作用。
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2. **文献名称**: *"Structural Insights into PFKP Regulation: Cryo-EM Analysis of Recombinant PFKP Tetramer Complex"*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜技术解析了重组PFKP蛋白的四聚体结构，揭示了其变构调节位点及与底物结合的关键氨基酸残基，为靶向PFKP的代谢疾病治疗提供了结构基础。
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3. **文献名称**: *"PFKP Reprograms Glucose Metabolism in Cancer Cells via Interaction with HIF-1α: Evidence from Recombinant Protein Assays"*
**作者**: Lee S, et al.
**摘要**: 研究利用重组PFKP蛋白及癌细胞模型，发现PFKP通过结合缺氧诱导因子HIF-1α增强糖酵解活性，促进肿瘤细胞在低氧环境下的增殖，提示其作为癌症治疗靶点的潜力。
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**提示**：实际文献检索建议使用PubMed或Google Scholar，以关键词“PFKP recombinant”、“PFKP expression and purification”或“PFKP structure/function”进行查询。
**Background of PFKP Recombinant Protein**
Phosphofructokinase, platelet type (PFKP), is a critical enzyme in glycolysis, catalyzing the phosphorylation of fructose-6-phosphate to fructose-1.6-bisphosphate—a rate-limiting step that regulates glycolytic flux. As one of three PFK isoforms in humans (PFKP, PFKM, and PFKL), PFKP is predominantly expressed in platelets, brain, and placenta, though it also plays tissue-specific roles in metabolic regulation. Its activity is tightly controlled by allosteric effectors (e.g., ATP, citrate, AMP) and hormonal signaling, linking cellular energy status to glycolytic output. Dysregulation of PFKP has been implicated in metabolic disorders, cancer (via the Warburg effect), and neurodegenerative diseases.
Recombinant PFKP proteins are engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional enzymes for research. These proteins retain catalytic activity and regulatory properties, enabling studies on PFKP’s structure-function relationships, metabolic interactions, and mutations linked to diseases. Applications include *in vitro* assays to screen metabolic modulators, investigate isoform-specific roles, or explore PFKP’s interplay with signaling pathways like HIF-1α or mTOR in cancer.
The development of PFKP recombinant proteins has advanced drug discovery and mechanistic studies, offering insights into targeting glycolysis for therapeutic interventions. Challenges remain in mimicking post-translational modifications *in vitro*, but ongoing refinements in expression systems continue to enhance their physiological relevance. Overall, PFKP recombinant tools are vital for dissecting metabolic reprogramming in health and disease.
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