| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAMTS16 |
| Uniprot No | Q8TE57 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1224aa |
| 氨基酸序列 | MKPRARGWRGLAALWMLLAQVAEQAPACAMGPAAAAPGSPSVPRPPPPAERPGWMEKGEYDLVSAYEVDHRGDYVSHEIMHHQRRRRAVPVSEVESLHLRLKGSRHDFHMDLRTSSSLVAPGFIVQTLGKTGTKSVQTLPPEDFCFYQGSLRSHRNSSVALSTCQGLSGMIRTEEADYFLRPLPSHLSWKLGRAAQGSSPSHVLYKRSTEPHAPGASEVLVTSRTWELAHQPLHSSDLRLGLPQKQHFCGRRKKYMPQPPKEDLFILPDEYKSCLRHKRSLLRSHRNEELNVETLVVVDKKMMQNHGHENITTYVLTILNMVSALFKDGTIGGNINIAIVGLILLEDEQPGLVISHHADHTLSSFCQWQSGLMGKDGTRHDHAILLTGLDICSWKNEPCDTLGFAPISGMCSKYRSCTINEDTGLGLAFTIAHESGHNFGMIHDGEGNMCKKSEGNIMSPTLAGRNGVFSWSPCSRQYLHKFLSTAQAICLADQPKPVKEYKYPEKLPGELYDANTQCKWQFGEKAKLCMLDFKKDICKALWCHRIGRKCETKFMPAAEGTICGHDMWCRGGQCVKYGDEGPKPTHGHWSDWSSWSPCSRTCGGGVSHRSRLCTNPKPSHGGKFCEGSTRTLKLCNSQKCPRDSVDFRAAQCAEHNSRRFRGRHYKWKPYTQVEDQDLCKLYCIAEGFDFFFSLSNKVKDGTPCSEDSRNVCIDGICERVGCDNVLGSDAVEDVCGVCNGNNSACTIHRGLYTKHHHTNQYYHMVTIPSGARSIRIYEMNVSTSYISVRNALRRYYLNGHWTVDWPGRYKFSGTTFDYRRSYNEPENLIATGPTNETLIVELLFQGRNPGVAWEYSMPRLGTEKQPPAQPSYTWAIVRSECSVSCGGGQMTVREGCYRDLKFQVNMSFCNPKTRPVTGLVPCKVSACPPSWSVGNWSACSRTCGGGAQSRPVQCTRRVHYDSEPVPASLCPQPAPSSRQACNSQSCPPAWSAGPWAECSHTCGKGWRKRAVACKSTNPSARAQLLPDAVCTSEPKPRMHEACLLQRCHKPKKLQWLVSAWSQCSVTCERGTQKRFLKCAEKYVSGKYRELASKKCSHLPKPSLELERACAPLPCPRHPPFAAAGPSRGSWFASPWSQCTASCGGGVQTRSVQCLAGGRPASGCLLHQKPSASLACNTHFCPIAEKKDAFCKDYFHWCYLVPQHGMCSHKFYGKQCCKTCSKSNL |
| 预测分子量 | 136,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ADAMTS16重组蛋白的虚构参考文献示例(基于研究领域常见方向模拟)：
1. **文献名称**: "Recombinant ADAMTS16 protease inhibits tumor angiogenesis through cleavage of VEGF"
**作者**: Liu Y, et al.
**摘要**: 本研究成功表达并纯化了人源ADAMTS16重组蛋白，证实其通过切割血管内皮生长因子(VEGF)抑制肿瘤血管生成，为癌症治疗提供潜在靶点。
2. **文献名称**: "Structural characterization of ADAMTS16 reveals a substrate-binding exosite in its ancillary domain"
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析ADAMTS16重组蛋白结构，发现其辅助结构域存在底物结合外显位点，揭示了该蛋白酶在细胞外基质重塑中的特异性作用机制。
3. **文献名称**: "ADAMTS16 regulates cardiac development via modulation of TGF-β signaling"
**作者**: Zhang H, et al.
**摘要**: 利用重组ADAMTS16蛋白进行功能实验，证明其通过调节TGF-β信号通路影响心肌细胞分化，提示其在先天性心脏病中的潜在病理作用。
4. **文献名称**: "ADAMTS16 polymorphisms and recombinant protein activity in polycystic ovary syndrome"
**作者**: Gupta R, et al.
**摘要**: 分析ADAMTS16基因多态性与重组蛋白酶活性关联，发现其与卵巢卵泡膜细胞纤维化异常相关，为多囊卵巢综合征的分子机制提供新见解。
注：以上文献为领域典型研究方向模拟，实际文献需通过PubMed/Web of Science等数据库检索真实发表论文。
ADAMTS16 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 16) is a member of the ADAMTS family of extracellular proteases, known for their roles in extracellular matrix (ECM) remodeling, tissue development, and disease processes. This multidomain enzyme contains a conserved catalytic metalloproteinase domain, a disintegrin-like module, a thrombospondin type-1 repeat (TSR) domain, and a unique C-terminal ancillary domain that mediates substrate recognition and cellular interactions. Unlike some well-characterized ADAMTS members (e.g., ADAMTS13 or ADAMTS5), ADAMTS16 remains understudied, though emerging evidence links it to organogenesis, cancer, and cardiovascular and reproductive disorders.
Recombinant ADAMTS16 protein is typically produced in mammalian or insect expression systems to ensure proper post-translational modifications, such as glycosylation, which are critical for its structural stability and enzymatic activity. The purified protein enables functional studies to elucidate its substrate specificity, proteolytic mechanisms, and regulatory pathways. Known substrates include ECM components like proteoglycans, though its full range of biological targets is still being explored. Research suggests ADAMTS16 may modulate TGF-β signaling and influence cell adhesion/migration, implicating it in fibrosis, tumor metastasis, and vascular remodeling. Dysregulation of ADAMTS16 has been observed in cancers (e.g., breast, prostate) and polycystic ovarian syndrome, highlighting its potential as a therapeutic target or biomarker. However, its precise physiological and pathological roles require further investigation. Recombinant ADAMTS16 tools (e.g., active enzymes, mutants, or inhibitory antibodies) are vital for dissecting its contributions to tissue homeostasis and disease, bridging gaps between genetic associations and mechanistic understanding.
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