| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HCK |
| Uniprot No | P49674 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416aa |
| 氨基酸序列 | MELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK |
| 预测分子量 | 49.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HCK重组蛋白的模拟参考文献(内容为示例，非真实文献)：
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1. **文献名称**: "Expression and Purification of Active Human HCK Tyrosine Kinase in E. coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功重组表达人源HCK激酶，优化纯化条件获得高活性蛋白，并验证其体外自磷酸化功能。
2. **文献名称**: "Structural Insights into HCK Kinase Regulation by SH3 Domain Interactions"
**作者**: Miller R, et al.
**摘要**: 通过X射线晶体学解析重组HCK蛋白的SH3结构域与底物结合机制，揭示其构象变化对激酶活性的调控作用。
3. **文献名称**: "HCK Recombinant Protein as a Therapeutic Target in Chronic Myeloid Leukemia"
**作者**: Gupta S, et al.
**摘要**: 利用重组HCK蛋白筛选小分子抑制剂，发现其对BCR-ABL耐药性白血病细胞株具有选择性抑制效果，提示潜在治疗价值。
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注：以上文献信息为模拟生成，实际引用需查询真实数据库(如PubMed)。
HCK (hematopoietic cell kinase) is a member of the SRC family of non-receptor tyrosine kinases, primarily expressed in hematopoietic cells, particularly myeloid cells like macrophages, neutrophils, and dendritic cells. It plays a critical role in immune response regulation, including phagocytosis, cytokine production, and cell migration. HCK is activated by various extracellular signals, such as integrin engagement and immune complex binding, which trigger phosphorylation cascades to modulate intracellular signaling pathways like PI3K/AKT, MAPK, and STAT. Dysregulation of HCK has been implicated in several diseases, including chronic myeloid leukemia, autoimmune disorders, and inflammatory conditions, making it a potential therapeutic target.
Recombinant HCK proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional kinase domains or full-length proteins for research and drug discovery. These proteins retain catalytic activity and are utilized in kinase activity assays, inhibitor screening, and structural studies. HCK's dual regulatory domains (SH3 and SH2) and autophosphorylation sites are often preserved in recombinant forms to mimic native conformational changes. Researchers employ these proteins to study HCK's role in immune cell signaling, its interaction with substrates like CBL and FAK, and its cross-talk with oncogenic pathways. Recent interest in HCK inhibitors for cancer immunotherapy has further driven demand for high-quality recombinant HCK in preclinical validation. Its unique activation mechanism, involving membrane translocation and phosphorylation at Tyr411. makes it a model for studying SRC kinase dynamics in health and disease.
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