| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HEMGN |
| Uniprot No | Q9BXL5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-484aa |
| 氨基酸序列 | MDLGKDQSHL KHHQTPDPHQ EENHSPEVIG TWSLRNRELL RKRKAEVHEK ETSQWLFGEQ KKRKQQRTGK GNRRGRKRQQ NTELKVEPQP QIEKEIVEKA LAPIEKKTEP PGSITKVFPS VASPQKVVPE EHFSEICQES NIYQENFSEY QEIAVQNHSS ETCQHVSEPE DLSPKMYQEI SVLQDNSSKI CQDMKEPEDN SPNTCQVISV IQDHPFKMYQ DMAKREDLAP KMCQEAAVPK ILPCPTSEDT ADLAGCSLQA YPKPDVPKGY ILDTDQNPAE PEEYNETDQG IAETEGLFPK IQEIAEPKDL STKTHQESAE PKYLPHKTCN EIIVPKAPSH KTIQETPHSE DYSIEINQET PGSEKYSPET YQEIPGLEEY SPEIYQETSQ LEEYSPEIYQ ETPGPEDLST ETYKNKDVPK ECFPEPHQET GGPQGQDPKA HQEDAKDAYT FPQEMKEKPK EEPGIPAILN ESHPENDVYS YVLF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HEMGN(Hemogen)重组蛋白的3篇文献示例(内容为虚构概括，仅供参考)：
1. **文献名称**: "Recombinant HEMGN Promotes Erythroid Differentiation via BMP Signaling"
**作者**: Zhang L, et al.
**摘要**: 研究通过体外重组表达HEMGN蛋白，发现其通过激活BMP/Smad通路促进红细胞分化，为贫血治疗提供潜在靶点。
2. **文献名称**: "Structural and Functional Analysis of Hemogen in Zebrafish Hematopoiesis"
**作者**: Smith JR, Johnson M.
**摘要**: 利用重组HEMGN蛋白进行斑马鱼模型实验，揭示了其在早期造血干细胞增殖中的关键作用及蛋白质结构功能域。
3. **文献名称**: "HEMGN Recombinant Protein Suppresses Tumor Growth in Leukemia Models"
**作者**: Wang Y, et al.
**摘要**: 发现重组HEMGN通过调控细胞周期蛋白抑制白血病细胞增殖，并在小鼠模型中显著降低肿瘤负荷。
(注：以上文献信息为模拟示例，实际研究需通过PubMed或Google Scholar等平台检索真实发表的论文。)
**Background of HEMGN Recombinant Protein**
HEMGN (Hemogen), also known as EDAG (embryonic development-associated gene), is a nuclear protein encoded by the *HEMGN* gene, primarily expressed in hematopoietic tissues and embryonic stem cells. It plays a critical role in regulating hematopoiesis, particularly during erythroid (red blood cell) differentiation and proliferation. Structurally, HEMGN contains a conserved N-terminal domain and a C-terminal proline-rich region, which may mediate protein-protein interactions and transcriptional regulation.
The recombinant HEMGN protein is engineered using molecular cloning techniques, where the *HEMGN* gene is inserted into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to produce purified, functional protein. This recombinant form retains biological activity, enabling researchers to study its mechanisms in vitro and in vivo. Studies suggest HEMGN interacts with transcriptional regulators (e.g., GATA-1. KLF1) and signaling pathways (e.g., BMP/Smad) to modulate gene expression critical for blood cell development.
Aberrant HEMGN expression has been linked to hematologic disorders and cancers. Overexpression is observed in certain leukemias, implying a potential oncogenic role, while its downregulation may impair erythropoiesis. Recombinant HEMGN is thus a valuable tool for investigating hematopoiesis, drug screening for blood-related diseases, and exploring therapeutic strategies targeting hematopoietic stem cells or malignancies.
In summary, HEMGN recombinant protein serves as a key reagent for dissecting molecular pathways in blood cell formation and disease, bridging basic research with translational applications in hematology and oncology.
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