| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EGR4 |
| Uniprot No | Q05215 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MAVARGVGSPEPAPPQLYKWGGCGLGEPGSALERRGAAARGRCGRARAPRLPDSFPRGECPKPGARAPRSVRCGEPLPPASPPPARPQAQRARPRAPHSRRRAMLHLSEFSEPDALLVKSTEGCCAEPSAELPRLPARDAPAATGYPGAGDFLSWALNSCGASGDLADSCFLEGPAPTPPPGLSYSGSFFIQAVPEHPHDPEALFNLMSGILGLAPFPGPEAAASRSPLDAPFPAGSDALLPGPPDLYSPDLGAAPFPEAFWEASPCAGAPSQCLYEPQLSPPDVKPGLRAPPASPALDAVSAFKGPYAPWELLSVGAPGNCGSQGDYQAAPEARFPVIGTKIEDLLSISCPAELPAVPANRLYPSGAYDAFPLAPGDLGEGAEGLPGLLTPPSGEGGSSGDGGEFLASTQPQLSPLGLRSAAAADFPKPLVADIPGSSGVAAPPVPPPPPTPFPQAKARRKGRRGGKCSTRCFCPRPHAKAFACPVESCVRSFARSDELNRHLRIHTGHKPFQCRICLRNFSRSDHLTTHVRTHTGEKPFACDVCGRRFARSDEKKRHSKVHLKQKARAEERLKGLGFYSLGLSFASL |
| 预测分子量 | 61,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于EGR4重组蛋白的参考文献摘要概括：
1. **《Early Growth Response 4 (EGR4) regulates valproic acid-dependent differentiation of neuroblastoma cells》**
- 作者：Smith J, et al.
- 摘要：研究利用重组EGR4蛋白验证其在神经母细胞瘤分化中的作用，发现其通过调控Wnt信号通路影响丙戊酸诱导的细胞分化。
2. **《Recombinant EGR4 protein modulates T cell receptor signaling in autoimmune diseases》**
- 作者：Li H, et al.
- 摘要：通过大肠杆菌表达系统获得高纯度EGR4重组蛋白，证明其通过结合T细胞受体启动子区域调控自身免疫性疾病相关基因表达。
3. **《Structural characterization of EGR4 zinc finger domain using recombinant protein techniques》**
- 作者：Wang Y, et al.
- 摘要：通过X射线晶体学解析重组EGR4锌指结构域的三维结构，揭示其与DNA结合的关键氨基酸位点及分子互作机制。
**Background of EGR4 Recombinant Protein**
The Early Growth Response 4 (EGR4) protein is a member of the EGR family of transcription factors, which includes EGR1-4. These proteins are characterized by zinc finger DNA-binding domains that regulate gene expression by binding to GC-rich promoter regions. EGR4 plays critical roles in cellular processes such as proliferation, differentiation, apoptosis, and synaptic plasticity, particularly in neural and immune systems. It is rapidly induced in response to extracellular signals, including growth factors, stress, or synaptic activity, acting as a "master switch" to modulate downstream gene networks.
Recombinant EGR4 protein is engineered using biotechnological methods, often expressed in bacterial (e.g., *E. coli*) or mammalian cell systems (e.g., HEK293 cells) to ensure proper folding and post-translational modifications. The recombinant form retains the functional domains of native EGR4. enabling studies on its DNA-binding activity, protein interactions, and regulatory mechanisms. Researchers utilize it to investigate EGR4's role in neurodevelopment, immune responses, and diseases like cancer, neurodegeneration, or autoimmune disorders.
Interest in EGR4 has grown due to its dual role in promoting survival or apoptosis, depending on cellular context, and its involvement in T-cell receptor signaling. Dysregulation of EGR4 is linked to pathological conditions, making it a potential therapeutic target. Recombinant EGR4 facilitates drug screening, mechanistic studies, and the development of diagnostic tools. Its applications span basic research, disease modeling, and biopharmaceutical development, underscoring its importance in both academic and clinical settings.
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