| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | EGR3 |
| Uniprot No | P43300 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-387aa |
| 氨基酸序列 | MTGKLAEKLP VTMSSLLNQL PDNLYPEEIP SALNLFSGSS DSVAHYNQMA TENVMDIGLT NEKPNPELSY SSSFQPAPGN KTVTYLGKFA FDSPSNWCQD NIISLMSAGI LGVPPASGAL STQTSTASMV QPPQGDVEAM YPALPPYSNC GDLYSEPVSF HDPQGNPGLA YSPQDYQSAK PALDSNLFPM IPDYNLYHHP NDMGSIPEHK PFQGMDPIRV NPPPITPLET IKAFKDKQIH PGFGSLPQPP LTLKPIRPRK YPNRPSKTPL HERPHACPAE GCDRRFSRSD ELTRHLRIHT GHKPFQCRIC MRSFSRSDHL TTHIRTHTGE KPFACEFCGR KFARSDERKR HAKIHLKQKE KKSEKGGAPS ASSAPTVSLA PVVTTCA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EGR3重组蛋白的3篇代表性文献摘要：
1. **标题**：Recombinant EGR3 Protein Regulates T Cell Activation and Autoimmune Inflammation
**作者**：Zhang Y, et al.
**摘要**：研究通过大肠杆菌表达重组EGR3蛋白，发现其通过调控IL-17等细胞因子表达，影响Th17细胞分化，揭示了EGR3在自身免疫性疾病中的潜在作用机制。
2. **标题**：Purification and Functional Analysis of EGR3 Recombinant Protein in Neuronal Differentiation
**作者**：Lee S, et al.
**摘要**：利用杆状病毒表达系统纯化EGR3重组蛋白，证实其与特定DNA序列结合并激活神经生长因子通路，为神经退行性疾病研究提供工具。
3. **标题**：Structural Characterization of EGR3 Zinc Finger Domain Using Recombinant Protein
**作者**：Wang H, et al.
**摘要**：通过X射线晶体学解析EGR3锌指结构域的三维结构，阐明其与靶基因启动子结合的分子机制，为设计调控EGR3活性的化合物奠定基础。
注：上述文献为示例，实际引用时建议通过PubMed或Web of Science以“EGR3 recombinant protein”为关键词检索最新研究。
Early growth response protein 3 (EGR3) is a zinc-finger transcription factor belonging to the EGR family, which plays critical roles in regulating gene expression during cellular proliferation, differentiation, and stress responses. EGR3 is encoded by the EGR3 gene and functions as an immediate-early gene product, rapidly induced by extracellular signals such as growth factors, neurotransmitters, or cellular stress. It binds to GC-rich promoter regions of target genes, modulating processes like neuronal development, immune regulation, and synaptic plasticity. Dysregulation of EGR3 has been linked to neurological disorders, schizophrenia, and cancer.
Recombinant EGR3 protein is produced through genetic engineering techniques, typically by expressing the EGR3 gene in bacterial (e.g., E. coli) or mammalian expression systems. This allows large-scale production of purified, functional EGR3 protein for research and therapeutic applications. The recombinant form retains the DNA-binding capacity and transcriptional regulatory functions of native EGR3. enabling studies on its molecular interactions, downstream signaling pathways, and role in disease mechanisms.
In research, recombinant EGR3 is widely used to investigate its involvement in T-cell activation, neuronal survival, and circadian rhythm regulation. It also serves as a tool to explore therapeutic strategies targeting EGR3-associated pathways, such as neuroprotection or immune modulation. Additionally, it aids in developing diagnostic assays to measure EGR3 levels in clinical samples. Despite progress, challenges remain in understanding its tissue-specific roles and post-translational modifications, driving ongoing studies to harness its potential in biomedicine.
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