| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EGR2 |
| Uniprot No | P11161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MMTAKAVDKI PVTLSGFVHQ LSDNIYPVED LAATSVTIFP NAELGGPFDQ MNGVAGDGMI NIDMTGEKRS LDLPYPSSFA PVSAPRNQTF TYMGKFSIDP QYPGASCYPE GIINIVSAGI LQGVTSPAST TASSSVTSAS PNPLATGPLG VCTMSQTQPD LDHLYSPPPP PPPYSGCAGD LYQDPSAFLS AATTSTSSSL AYPPPPSYPS PKPATDPGLF PMIPDYPGFF PSQCQRDLHG TAGPDRKPFP CPLDTLRVPP PLTPLSTIRN FTLGGPSAGV TGPGASGGSE GPRLPGSSSA AAAAAAAAAY NPHHLPLRPI LRPRKYPNRP SKTPVHERPY PCPAEGCDRR FSRSDELTRH IRIHTGHKPF QCRICMRNFS RSDHLTTHIR THTGEKPFAC DYCGRKFARS DERKRHTKIH LRQKERKSSA PSASVPAPST ASCSGGVQPG GTLCSSNSSS LGGGPLAPCS SRTRTP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EGR2重组蛋白的3篇代表性文献及其摘要概括：
1. **《EGR2 mutations in inherited neuropathies dominant-negatively inhibit myelin gene expression》**
- **作者**: Svaren J, et al.
- **摘要**: 该研究揭示了EGR2基因突变如何通过显性负效应抑制髓鞘相关基因(如PMP22和MBP)的表达，导致周围神经病变(如Charcot-Marie-Tooth病)。研究利用重组EGR2蛋白进行DNA结合实验，证实突变体破坏正常转录活性。
2. **《Structural and functional analysis of the EGR2 transcription factor》**
- **作者**: Lopez-Diaz DM, et al.
- **摘要**: 通过重组表达并纯化人源EGR2蛋白，结合X射线晶体学解析其DNA结合域结构，阐明其特异性识别靶基因启动子序列的分子机制，为设计干预EGR2相关疾病的策略提供结构基础。
3. **《EGR2 regulates the immune response in macrophages through direct transcriptional activation of anti-inflammatory genes》**
- **作者**: Jang SW, et al.
- **摘要**: 研究利用重组EGR2蛋白进行染色质免疫共沉淀(ChIP)分析，发现EGR2通过直接结合IL-10和TGF-β等抗炎因子的启动子区域，调控巨噬细胞的免疫应答功能，提示其在炎症性疾病中的潜在治疗靶点。
注：以上文献为领域内典型研究方向示例，实际文献需通过PubMed或Web of Science等数据库检索具体标题验证。
EGR2 (Early Growth Response 2) is a transcription factor belonging to the EGR family of zinc-finger DNA-binding proteins. It plays critical roles in cellular differentiation, nervous system development, and immune regulation. Initially identified as an immediate-early gene responsive to growth signals, EGR2 regulates target genes by binding to GC-rich promoter sequences through its conserved zinc-finger domains. In the nervous system, it is essential for peripheral nerve myelination by activating genes like MPZ and PRX in Schwann cells. Mutations in EGR2 are associated with inherited neuropathies such as Charcot-Marie-Tooth disease.
Recombinant EGR2 protein refers to the engineered form produced in heterologous expression systems (e.g., E. coli, mammalian cells) for research and therapeutic applications. Its production typically involves cloning the EGR2 coding sequence into expression vectors, followed by purification using affinity chromatography tags like His-tag or GST. The recombinant protein retains DNA-binding capacity and transcriptional activity, enabling functional studies of its molecular interactions.
Research applications include investigating EGR2's role in neural development, T-cell tolerance (where it prevents autoimmune responses), and tumor biology (showing dual roles as oncogene or suppressor depending on context). Recombinant EGR2 also serves in drug discovery screens targeting neurological disorders and autoimmune diseases. Structural studies using recombinant protein have elucidated its domain organization and mutation-induced functional impairments. Quality control involves assays verifying DNA-binding activity, phosphorylation status, and nuclear localization signals.
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