| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IHH |
| Uniprot No | Q14623 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-202aa |
| 氨基酸序列 | IIGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSE RFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVK LRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFD WVYYESKAHVHCSVKSEHSAAAKTGG |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IHH重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，实际引用时请核实真实来源)：
1. **《Recombinant Indian Hedgehog (IHH) Production in E. coli and Its Role in Chondrocyte Differentiation》**
- 作者：Smith A, et al.
- 摘要：研究报道了在大肠杆菌系统中高效表达IHH重组蛋白的优化方法，并验证其通过激活Hedgehog信号通路促进软骨细胞分化的功能，为骨组织工程研究提供工具。
2. **《Purification and Functional Characterization of Human IHH for Therapeutic Applications in Osteoarthritis》**
- 作者：Chen L, et al.
- 摘要：通过哺乳动物细胞表达系统纯化人源IHH蛋白，证实其在体外模型中缓解软骨退化，并探讨其作为骨关节炎治疗药物的潜力。
3. **《Structural Analysis of IHH Signaling Complex Using Recombinant Protein Crystallography》**
- 作者：Wang X, et al.
- 摘要：利用重组IHH蛋白解析其与受体Patched1的复合物晶体结构，揭示IHH介导信号转导的分子机制，为靶向Hedgehog通路药物设计提供结构基础。
**注意**：以上文献信息为示例性质，建议通过PubMed或Web of Science等平台检索真实文献，关键词如“Recombinant Indian Hedgehog”、“IHH protein expression”等。
**Background of IHH Recombinant Protein**
The Indian Hedgehog (IHH) protein is a member of the Hedgehog (Hh) signaling pathway, a critical regulator of embryonic development, tissue homeostasis, and cellular differentiation. IHH, initially identified for its role in skeletal development, is one of three mammalian Hedgehog paralogs, alongside Sonic Hedgehog (SHH) and Desert Hedgehog (DHH). It is secreted as a signaling molecule that binds to the Patched (PTCH) receptor, relieving its inhibition on the Smoothened (SMO) receptor and activating downstream transcription factors like GLI.
Recombinant IHH protein is engineered using biotechnological methods to produce a purified, bioactive form of the native protein. This involves expressing the *IHH* gene in heterologous systems, such as bacterial, insect, or mammalian cell lines, followed by purification to ensure high specificity and activity. The recombinant protein retains key structural features, including an N-terminal signaling domain responsible for receptor interaction and a C-terminal autoprocessing domain involved in lipid modification, which enhances its stability and functional integration into cellular membranes.
Functionally, IHH is essential for endochondral ossification, regulating chondrocyte proliferation, differentiation, and osteoblast activity. Mutations in IHH are linked to skeletal disorders like brachydactyly type A1 and craniofacial abnormalities. Beyond development, IHH dysregulation is implicated in cancers, fibrosis, and metabolic diseases.
Recombinant IHH is widely used in research to dissect Hedgehog pathway mechanisms, model diseases, and screen therapeutic agents targeting Hh-driven pathologies. Its standardized production ensures reproducibility in studies exploring skeletal biology, tissue regeneration, and oncogenesis. By enabling precise control over Hh signaling, recombinant IHH serves as a vital tool for both basic research and translational applications.
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