| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AP |
| Uniprot No | Q9H4A4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-650aa |
| 氨基酸序列 | MASGEHSPGSGAARRPLHSAQAVDVASASNFRAFELLHLHLDLRAEFGPP GPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGS EEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGV CWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDG FTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVW AEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGM ENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGF TMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIE PGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSI LADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLNTPGWPPYLPDLSPGDS LMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPP GNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGK QKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQIVAPKGS |
| 预测分子量 | 99 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AP(碱性磷酸酶)重组蛋白的3篇参考文献示例，涵盖不同研究方向：
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1. **文献名称**：*High-yield Production and Purification of Recombinant Alkaline Phosphatase in E. coli*
**作者**：Smith J. et al.
**摘要**：研究报道了利用大肠杆菌表达系统高效生产His标签重组碱性磷酸酶的方法，通过优化诱导条件和镍柱亲和层析纯化，获得高纯度酶制剂，并验证其催化活性与天然酶相当。
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2. **文献名称**：*Structural Insights into Recombinant Alkaline Phosphatase by X-ray Crystallography*
**作者**：Chen L. et al.
**摘要**：通过X射线晶体学解析重组碱性磷酸酶的三维结构，揭示了其活性中心的关键氨基酸残基，并探讨了突变体对酶活性的影响，为理性设计功能改良酶提供依据。
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3. **文献名称**：*Application of Recombinant AP in Phosphate Detection Biosensors*
**作者**：Wang Y. et al.
**摘要**：开发了一种基于重组碱性磷酸酶的新型生物传感器，用于快速检测水样中的磷酸盐浓度，验证了其在环境监测中的高灵敏度和稳定性。
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**注**：以上文献信息为模拟示例，实际引用时需根据具体研究方向检索真实文献(推荐数据库：PubMed、Web of Science)。
**Background of AP Recombinant Proteins**
Alkaline phosphatase (AP) recombinant proteins are engineered fusion proteins that combine the functional domains of alkaline phosphatase with other proteins or peptides, leveraging recombinant DNA technology. AP, a hydrolase enzyme that removes phosphate groups from nucleotides, proteins, and other molecules, is widely utilized in molecular and cellular biology due to its stability, ease of detection, and compatibility with diverse experimental systems. The development of AP recombinant proteins emerged in the 1980s alongside advances in genetic engineering, enabling the fusion of AP with target proteins to create reporter molecules for tracking gene expression, protein localization, and ligand-receptor interactions.
A key application lies in *in situ* hybridization and immunohistochemistry, where AP-tagged probes or antibodies enable sensitive detection via chromogenic or chemiluminescent substrates. AP’s thermal stability and resistance to inactivation by detergents make it ideal for high-throughput assays, such as ELISA and phage display. Additionally, AP fusion proteins are employed in studying protein secretion and membrane anchoring, as AP’s enzymatic activity is retained only when properly folded and transported.
The commercial availability of AP cloning vectors (e.g., pAPtag vectors) further streamlined the creation of fusion constructs. These tools facilitated breakthroughs in understanding cell signaling, protein trafficking, and extracellular interactions. Despite competition from fluorescent proteins (e.g., GFP), AP remains relevant due to its cost-effectiveness, compatibility with archival tissues, and quantitative enzymatic readouts. Recent innovations include engineered AP variants with enhanced brightness or altered substrate specificity, expanding their utility in multiplexed assays and live-cell imaging. Overall, AP recombinant proteins continue to serve as versatile tools bridging structural analysis and functional genomics.
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