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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHI3L1 |
| Uniprot No | P36222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-383aa |
| 氨基酸序列 | YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEW NDVTLYGMLN TLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTF IKSVPPFLRTHGFDGLDLAWLY PGRRDKQHFTTLIKEMKAEFIKEAQP GKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFI SIMTYDFHGAWRGT TGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTF GR SFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILG QQVPYATK GNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQG SFCGQDLRFPLTNAIKDALA AT |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Role of chitinase 3-like-1 in T-helper type 2 inflammation and allergic airway remodeling"**
- 作者：Lee C.G. et al.
- 摘要：研究通过重组CHI3L1蛋白在小鼠哮喘模型中验证其促炎和促纤维化作用，发现其通过激活MAPK信号通路增强Th2炎症反应及气道重塑。
2. **"Chitinase 3-like-1 regulates macrophage and hepatic stellate cell activation via Nrf2 signaling in liver fibrosis"**
- 作者：Chen C.C. et al.
- 摘要：利用重组CHI3L1蛋白体外处理肝星状细胞和巨噬细胞，揭示其通过激活Nrf2通路促进肝纤维化进程，为治疗靶点提供依据。
3. **"Chitinase 3-like-1 contributes to IL-8 expression in human articular chondrocytes"**
- 作者：Recklies A.D. et al.
- 摘要：通过重组CHI3L1蛋白刺激人软骨细胞，证实其上调IL-8等炎症因子表达，提示其在骨关节炎病理机制中的潜在作用。
4. **"High serum YKL-40 levels in patients with primary breast cancer predict poor prognosis"**
- 作者：Johansen J.S. et al.
- 摘要：结合重组蛋白实验与临床数据，发现CHI3L1通过促进肿瘤微环境中的血管生成和侵袭，与乳腺癌患者不良预后显著相关。
CHI3L1 (Chitinase-3-like protein 1), also known as YKL-40 or HC-gp39. is a secreted glycoprotein belonging to the glycosyl hydrolase 18 family. Unlike true chitinases, CHI3L1 lacks enzymatic activity due to mutations in its catalytic domain but retains chitin-binding properties. It is evolutionarily conserved and widely expressed in mammals, primarily by immune cells (macrophages, neutrophils), epithelial cells, and activated chondrocytes. Its expression is upregulated during inflammation, tissue remodeling, and cellular stress, making it a biomarker for various pathological conditions.
Structurally, CHI3L1 comprises a conserved N-terminal chitin-binding domain and a C-terminal heparin-binding domain, enabling interactions with extracellular matrix components and cell-surface receptors like IL-13Rα2. syndecan-1. and RAGE. These interactions activate signaling pathways (e.g., MAPK, AKT, Wnt/β-catenin) to regulate cell proliferation, differentiation, apoptosis, and angiogenesis. CHI3L1 plays dual roles: promoting tissue repair in acute injury while driving fibrosis and cancer progression in chronic diseases.
Recombinant CHI3L1 proteins are produced using expression systems (e.g., E. coli, mammalian cells) for functional studies. They retain biological activity and are used to investigate CHI3L1's role in diseases such as asthma, rheumatoid arthritis, liver fibrosis, Alzheimer’s disease, and cancers (glioblastoma, breast cancer). Elevated serum CHI3L1 correlates with poor prognosis in cancer and severe inflammation, highlighting its clinical relevance. Recent studies also implicate it in COVID-19 cytokine storms and metabolic disorders.
Research on recombinant CHI3L1 aids in developing therapeutic strategies, including neutralizing antibodies and small-molecule inhibitors, to modulate its pathological effects while preserving its protective functions.
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