| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LPIN1 |
| Uniprot No | Q14693 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-890aa |
| 氨基酸序列 | MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVIAPSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQDDIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LPIN1重组蛋白的3篇文献及其摘要的简要概括：
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1. **文献名称**：*"Recombinant expression and functional characterization of human LPIN1 as a phosphatidic acid phosphatase"*
**作者**：Smith J, et al.
**摘要内容**：本研究报道了人源LPIN1基因在大肠杆菌中的重组表达及纯化。作者通过构建His标签融合蛋白，利用镍柱层析获得高纯度LPIN1.并验证其作为磷酸酸磷酸酶(PAP)的活性，证实其在甘油三酯合成中的关键作用。
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2. **文献名称**：*"Structural insights into LPIN1-mediated lipid metabolism by cryo-EM analysis of the recombinant protein"*
**作者**：Chen L, et al.
**摘要内容**：该研究通过冷冻电镜解析了重组表达的小鼠LPIN1蛋白三维结构，揭示了其与磷脂酸底物结合的关键结构域，并探讨了突变对脂代谢相关疾病(如脂肪营养不良)的分子机制影响。
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3. **文献名称**：*"Optimization of LPIN1 expression in mammalian cells for studying its role in insulin resistance"*
**作者**：Wang Y, et al.
**摘要内容**：作者在HEK293细胞中重组表达了人LPIN1蛋白，优化了表达条件以提高产量。研究表明，过表达LPIN1可改善胰岛素信号通路，提示其在代谢综合征中的潜在治疗价值。
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注：以上文献信息为模拟示例，实际文献需通过数据库(如PubMed、Google Scholar)检索确认。建议使用关键词“LPIN1 recombinant expression”或结合具体研究领域进一步筛选。
**Background of LPIN1 Recombinant Protein**
LPIN1 encodes a phosphatidic acid phosphatase (PAP) enzyme critical for lipid metabolism, particularly in triglyceride and phospholipid synthesis. It regulates the conversion of phosphatidic acid to diacylglycerol, a key step in glycerolipid biosynthesis. LPIN1 also interacts with transcription factors like PGC-1α and PPARs, influencing fatty acid oxidation and mitochondrial biogenesis, thus bridging lipid storage and energy utilization.
Mutations in *LPIN1* are linked to human disorders, including recurrent rhabdomyolysis, acute myopathy, and metabolic syndrome. Its role in lipid homeostasis extends to metabolic tissues such as liver, adipose, and skeletal muscle, making it a focal point in obesity, diabetes, and cardiovascular disease research.
Recombinant LPIN1 proteins are engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its enzymatic activity, structure-function relationships, and interactions. These proteins are vital for *in vitro* assays, drug screening, and disease modeling. Tagged versions (e.g., His-tag) facilitate purification and detection, enabling mechanistic insights into lipid disorders.
Current research leverages LPIN1 recombinant proteins to explore therapeutic strategies, including enzyme replacement or modulation of its regulatory pathways. Their application enhances understanding of metabolic diseases and supports development of targeted therapies.
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