| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FADS1 |
| Uniprot No | O60427 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MAPDPVAAETAAQGPTPRYFTWDEVAQRSGCEERWLVIDRKVYNISEFTRRHPGGSRVISHYAGQDATDPFVAFHINKGLVKKYMNSLLIGELSPEQPSFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGTSFLPFLLCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMHFQHHAKPNCFRKDPDINMHPFFFALGKILSVELGKQKKKYMPYNHQHKYFFLIGPPALLPLYFQWYIFYFVIQRKKWVDLAWMITFYVRFFLTYVPLLGLKAFLGLFFIVRFLESNWFVWVTQMNHIPMHIDHDRNMDWVSTQLQATCNVHKSAFNDWFSGHLNFQIEHHLFPTMPRHNYHKVAPLVQSLCAKHGIEYQSKPLLSAFADIIHSLKESGQLWLDAYLHQ |
| 预测分子量 | 51,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FADS1重组蛋白的参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索获取)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human FADS1 in Mammalian Cells*
**作者**：Smith A, et al.
**摘要**：本研究在HEK293细胞中成功表达了重组人FADS1蛋白，并通过亲和层析纯化。功能分析表明，重组蛋白具有Δ5去饱和酶活性，可将二高-γ-亚麻酸转化为花生四烯酸，为体外研究FADS1酶动力学提供了工具。
2. **文献名称**：*Crystal Structure of FADS1 Reveals Substrate-Binding Sites for Fatty Acid Desaturation*
**作者**：Chen L, et al.
**摘要**：通过昆虫细胞表达系统获得高纯度FADS1重组蛋白，利用X射线晶体学解析其三维结构，揭示了保守的组氨酸簇和底物结合口袋，阐明了催化机制及基因多态性对酶活性的潜在影响。
3. **文献名称**：*Functional Impact of FADS1 Genetic Variants on Recombinant Protein Activity*
**作者**：Wang Y, et al.
**摘要**：构建了携带不同SNP(如rs174547)的FADS1重组蛋白突变体，在大肠杆菌中表达并纯化。酶活实验显示特定突变导致Δ5去饱和效率下降，提示遗传变异与脂代谢疾病的关联机制。
4. **文献名称**：*Optimization of FADS1 Recombinant Production in Yeast for Industrial Applications*
**作者**：Kimura T, et al.
**摘要**：开发了一种基于毕赤酵母的重组FADS1高效表达系统，通过发酵优化使蛋白产量提升5倍。纯化蛋白可用于功能性食品添加剂开发，增强多不饱和脂肪酸的生物合成。
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建议通过PubMed或Web of Science搜索关键词“FADS1 recombinant expression”“FADS1 structure/function”获取真实文献。
The fatty acid desaturase 1 (FADS1) gene encodes a key enzyme in the biosynthesis of long-chain polyunsaturated fatty acids (PUFAs), which are critical for membrane structure, cell signaling, and inflammation regulation. Located on human chromosome 11q22.3. FADS1 catalyzes the introduction of double bonds into fatty acid chains, primarily converting linoleic acid (LA) and alpha-linolenic acid (ALA) into arachidonic acid (AA) and eicosapentaenoic acid (EPA), respectively. These products serve as precursors for bioactive lipid mediators, including prostaglandins and leukotrienes. Genetic variations in FADS1 are linked to altered PUFA levels and associated with metabolic disorders, cardiovascular diseases, and neurodevelopmental conditions.
Recombinant FADS1 protein is engineered for in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and regulatory interactions. Produced using expression systems like *E. coli* or mammalian cell cultures, the purified protein retains desaturase activity, enabling researchers to explore its role in lipid metabolism under controlled conditions. Its recombinant form is particularly valuable for high-throughput screening of potential inhibitors or modulators, which may have therapeutic implications for inflammation-related diseases or metabolic syndromes. Structural studies using recombinant FADS1 have also advanced understanding of its catalytic domain and membrane-binding regions, offering insights for drug design. Additionally, it aids in studying how genetic polymorphisms affect enzyme efficiency, contributing to personalized nutrition and pharmacogenomics research. Overall, FADS1 recombinant protein serves as a vital tool in bridging genetic, biochemical, and clinical studies of lipid-mediated health and disease.
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