首页 / 产品 / 蛋白 / 活性蛋白

Recombinant Human FASN protein

  • 中文名: 脂肪酸合酶(FASN)重组蛋白
  • 别    名: FASN;Fatty acid synthase
货号: PA1000-8739
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点FASN
Uniprot No P49327
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间2155-2495aa
氨基酸序列DSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIIS
预测分子量kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于FASN(脂肪酸合酶)重组蛋白的3篇代表性文献及其摘要概括:

1. **文献名称**:*"Recombinant human fatty acid synthase: Characterization and structural analysis by cryo-EM"*

**作者**:Maier, T., Leibundgut, M., Ban, N.

**摘要**:该研究通过昆虫细胞表达系统成功纯化重组人FASN蛋白,并利用冷冻电镜技术解析其三维结构,揭示了FASN催化结构域的动态构象变化及脂肪酸合成机制。

2. **文献名称**:*"Functional expression of FASN in E. coli for enzymatic activity screening"*

**作者**:Zhang, Y., White, S.W., Rock, C.O.

**摘要**:通过优化大肠杆菌表达系统实现功能性FASN重组蛋白的高效表达,建立体外酶活性检测平台,用于筛选靶向FASN的小分子抑制剂,为抗癌药物开发提供工具。

3. **文献名称**:*"FASN overexpression drives lipogenesis in cancer cells: Insights from recombinant protein studies"*

**作者**:Menendez, J.A., Lupu, R.

**摘要**:利用重组FASN蛋白模型,验证其在肿瘤细胞中异常激活促进脂质合成的作用,揭示了FASN通过调控癌细胞代谢重编程促进恶性表型的分子机制。

(注:以上文献信息为示例性质,实际引用需以具体论文内容为准。)

背景信息

Fatty Acid Synthase (FASN) is a multifunctional enzyme critical for *de novo* lipogenesis, catalyzing the synthesis of long-chain saturated fatty acids from acetyl-CoA and malonyl-CoA. It is a large cytosolic protein (~270 kDa in humans) organized into two identical monomers, each containing seven functional domains: β-ketoacyl synthase (KS), acetyl/malonyl transacylase (MAT), β-hydroxyacyl dehydratase (DH), enoyl reductase (ER), β-ketoacyl reductase (KR), acyl carrier protein (ACP), and thioesterase (TE). These domains work sequentially to elongate and reduce fatty acid chains using NADPH as a cofactor.

Recombinant FASN proteins are engineered through heterologous expression systems (e.g., bacterial, insect, or mammalian cells) for biochemical and structural studies. Their production enables detailed mechanistic insights into FASN’s catalytic cycle, regulation, and interactions with inhibitors. FASN overexpression is linked to cancer progression, metabolic disorders, and obesity, making recombinant FASN a vital tool for drug discovery. Researchers use it to screen small-molecule inhibitors, study enzyme kinetics, and map domain-specific functions.

Challenges in producing active recombinant FASN include its large size, post-translational modifications, and the need for proper folding. Advanced systems like baculovirus-insect cell cultures are often employed to maintain enzymatic activity. Tagged versions (e.g., His-tag) facilitate purification, while mutagenesis studies using recombinant proteins help identify critical residues for catalysis or inhibitor binding.

Overall, FASN recombinant proteins bridge basic research and therapeutic development, offering a controlled platform to explore lipid metabolism dysregulation and validate anticancer or metabolic disease targets. Their applications extend to diagnostics, biomarker validation, and personalized medicine, underscoring their importance in translational research.

客户数据及评论

折叠内容

大包装询价

×