| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIPH |
| Uniprot No | Q8WWY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-451aa |
| 氨基酸序列 | EE TCPSFTRLSF HSAVVGTGLN VRLMLYTRKN LTCAQTINSS AFGNLNVTKK TTFIVHGFRP TGSPPVWMDD LVKGLLSVED MNVVVVDWNR GATTLIYTHA SSKTRKVAMV LKEFIDQMLA EGASLDDIYM IGVSLGAHIS GFVGEMYDGW LGRITGLDPA GPLFNGKPHQ DRLDPSDAQF VDVIHSDTDA LGYKEPLGNI DFYPNGGLDQ PGCPKTILGG FQYFKCDHQR SVYLYLSSLR ESCTITAYPC DSYQDYRNGK CVSCGTSQKE SCPLLGYYAD NWKDHLRGKD PPMTKAFFDT AEESPFCMYH YFVDIITWNK NVRRGDITIK LRDKAGNTTE SKINHEPTTF QKYHQVSLLA RFNQDLDKVA AISLMFSTGS LIGPRYKLRI LRMKLRSLAH PERPQLCRYD LVLMENVETV FQPILCPELQ L |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LIPH重组蛋白的3篇参考文献的简要概括：
1. **文献名称**: *"Mutations in LIPH are responsible for autosomal recessive hypotrichosis"*
**作者**: Shimomura Y, et al. (2008)
**摘要**: 本研究通过基因测序发现LIPH基因突变导致常染色体隐性遗传性少毛症。研究者利用重组LIPH蛋白证实其编码的磷脂酶活性，揭示其在毛囊脂质代谢及毛发发育中的关键作用。
2. **文献名称**: *"LIPH contributes to skin barrier formation through lipid synthesis in keratinocytes"*
**作者**: Inoue A, et al. (2019)
**摘要**: 文章通过体外表达重组人源LIPH蛋白，发现其参与角质细胞中磷脂酰肌醇代谢，促进皮肤屏障相关脂质的合成，为LIPH缺失相关鱼鳞病的治疗提供分子机制依据。
3. **文献名称**: *"Functional characterization of recombinant LIPH protein in hair follicle regeneration"*
**作者**: Sugiura K, et al. (2012)
**摘要**: 研究利用杆状病毒系统表达重组LIPH蛋白，发现其水解磷脂酰肌醇生成信号分子lyso-PA，激活毛乳头细胞的增殖通路，证实LIPH在毛囊再生中的潜在应用价值。
(注：以上文献信息为基于领域知识的模拟概括，实际引用需核实具体文献内容。)
**Background of LIPH Recombinant Protein**
LIPH (Lipoma HMGIC Fusion Partner-Like Protein), also known as phosphatidic acid phosphatase type 2 domain-containing protein 3 (PPAPDC3), is a transmembrane protein encoded by the *LIPH* gene. It belongs to the phosphatidic acid phosphatase (PAP) family and plays a critical role in lipid metabolism and cell signaling. Structurally, LIPH contains a conserved PAP2 domain, which facilitates its enzymatic activity in hydrolyzing phosphatidic acid (PA) to diacylglycerol (DAG), a key lipid secondary messenger involved in cellular processes like membrane trafficking, proliferation, and differentiation.
LIPH gained attention due to its association with autosomal recessive hypotrichosis, a rare genetic disorder characterized by fragile hair and progressive hair loss. Mutations in *LIPH* disrupt the production of functional LIPH protein, impairing hair follicle integrity and sebaceous gland function. This highlights its role in maintaining epidermal homeostasis and hair follicle cycling. Additionally, LIPH is expressed in tissues such as the skin, prostate, and digestive tract, suggesting broader physiological roles in lipid-mediated signaling and cellular adhesion.
Recombinant LIPH protein is generated using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to produce purified, bioactive forms of the protein for research and therapeutic applications. Its recombinant form enables studies on lipid signaling pathways, disease mechanisms, and drug discovery. For instance, it aids in exploring LIPH’s interaction with lipid substrates or its regulatory effects on downstream targets like epidermal growth factor receptor (EGFR) pathways. Potential therapeutic strategies include using recombinant LIPH to restore lipid balance in genetic disorders or to modulate cell proliferation in cancers where LIPH expression is dysregulated.
Overall, LIPH recombinant protein serves as a vital tool for dissecting its biological functions and developing targeted interventions for related diseases.
×
