| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUC7 |
| Uniprot No | Q8TAX7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-377aa |
| 氨基酸序列 | RERDHELRHRRHHHQSPKSHFELPHYPGLLAHQKPFIRKSYKCLHKRCRPKLPPSPNNPPKFPNPHQPPKHPDKNSSVVNPTLVATTQIPSVTFPSASTKITTLPNVTFLPQNATTISSRENVNTSSSVATLAPVNSPAPQDTTAAPPTPSATTPAPPSSSAPPETTAAPPTPSATTQAPPSSSAPPETTAAPPTPPATTPAPPSSSAPPETTAAPPTPSATTPAPLSSSAPPETTAVPPTPSATTLDPSSASAPPETTAAPPTPSATTPAPPSSPAPQETTAAPITTPNSSPTTLAPDTSETSAAPTHQTTTSVTTQTTTTKQPTSAPGQNKISRFLLYMKNLLNRIIDDMVEQ |
| 预测分子量 | 95.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUC7重组蛋白研究的示例参考文献格式(注：以下为模拟示例，具体文献需根据实际数据库检索确认)：
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1. **文献名称**: *"Expression and purification of recombinant human MUC7 in Escherichia coli for antimicrobial studies"*
**作者**: Smith A, et al.
**摘要**: 研究报道了在大肠杆菌系统中成功表达重组人MUC7蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白，并证实其体外抑制口腔致病菌生长的活性。
2. **文献名称**: *"Structural characterization of recombinant MUC7 mucin domain and its role in biofilm inhibition"*
**作者**: Lee J, et al.
**摘要**: 利用昆虫细胞表达系统制备重组MUC7粘蛋白结构域，通过质谱和圆二色性分析其结构，发现其能有效破坏念珠菌生物膜形成。
3. **文献名称**: *"Recombinant MUC7 fusion proteins as potential therapeutic agents in oral mucositis"*
**作者**: Brown K, et al.
**摘要**: 构建MUC7与抗菌肽的融合蛋白，在哺乳动物细胞中表达，证明其增强的黏膜黏附性和对化疗后口腔溃疡的修复作用。
4. **文献名称**: *"Functional analysis of glycosylated recombinant MUC7 in salivary lubrication"*
**作者**: Zhang Y, et al.
**摘要**: 在CHO细胞中表达糖基化重组MUC7.模拟天然唾液粘蛋白的润滑功能，为开发人工唾液替代品提供实验依据。
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建议通过PubMed或Google Scholar以关键词“recombinant MUC7”、“MUC7 expression”检索最新文献获取真实数据。
MUC7. a member of the mucin protein family, is a small secreted glycoprotein predominantly expressed in salivary glands and present in oral secretions. It plays critical roles in mucosal immunity, lubrication, and microbial interactions. Structurally, MUC7 contains a central glycosylated domain rich in serine/threonine residues for O-linked glycan attachment, flanked by conserved N- and C-terminal regions. Its repetitive peptide backbone and heavy glycosylation contribute to viscoelastic properties, enabling it to trap pathogens, modulate bacterial adhesion, and protect epithelial surfaces.
The recombinant MUC7 protein, produced through genetic engineering in heterologous expression systems (e.g., bacterial, mammalian, or insect cells), allows researchers to study its structure-function relationships without isolating it from complex biological fluids. This approach overcomes challenges like low natural abundance and contamination from co-purified molecules. Recombinant technology also enables site-specific mutagenesis to dissect functional domains, such as its antimicrobial regions or glycosylation-dependent interactions.
Research on recombinant MUC7 has advanced understanding of its role in oral health, including dysbiosis-related diseases (e.g., caries, periodontitis) and systemic conditions like cystic fibrosis or cancer. It serves as a tool for developing diagnostic biomarkers, antimicrobial peptides, or synthetic mucin-inspired biomaterials. However, replicating its native glycosylation pattern remains challenging, as post-translational modifications significantly influence its biological activity. Current efforts focus on optimizing expression systems to mimic natural glycoforms while maintaining scalability for therapeutic applications.
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