纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MLZE |
Uniprot No | Q9BYG8 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-508aa |
氨基酸序列 | MPSMLERISKNLVKEIGSKDLTPVKYLLSATKLRQFVILRKKKDSRSSFWEQSDYVPVEFSLNDILEPSSSVLETVVTGPFHFSDIMIQKHKADMGVNVGIEVSVSGEASVDHGCSLEFQIVTIPSPNLEDFQKRKLLDPEPSFLKECRRRGDNLYVVTEAVELINNTVLYDSSSVNILGKIALWITYGKGQGQGESLRVKKKALTLQKGMVMAYKRKQLVIKEKAILISDDDEQRTFQDEYEISEMVGYCAARSEGLLPSFHTISPTLFNASSNDMKLKPELFLTQQFLSGHLPKYEQVHILPVGRIEEPFWQNFKHLQEEVFQKIKTLAQLSKDVQDVMFYSILAMLRDRGALQDLMNMLELDSSGHLDGPGGAILKKLQQDSNHAWFNPKDPILYLLEAIMVLSDFQHDLLACSMEKRILLQQQELVRSILEPNFRYPWSIPFTLKPELLAPLQSEGLAITYGLLEECGLRMELDNPRSTWDVEAKMPLSALYGTLSLLQQLAEA |
预测分子量 | 73.7kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MERS-CoV重组蛋白(可能与“MLZE”相关,推测为重组表达技术或疫苗研究)的3篇参考文献示例:
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1. **文献名称**: "A recombinant receptor-binding domain of MERS-CoV spike protein induces protective immunity in mice"
**作者**: Zhang, N., Channappanavar, R., Ma, C., et al.
**摘要**:研究利用昆虫细胞系统表达MERS-CoV刺突蛋白的受体结合域(RBD)重组蛋白,证明其在小鼠模型中能诱导中和抗体并有效抵抗病毒攻击。
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2. **文献名称**: "Baculovirus expression of the full-length spike protein of MERS-CoV generates strong immune responses in vaccinated animals"
**作者**: Wang, Y., Tai, W., Zhang, X., et al.
**摘要**:通过杆状病毒表达系统生产MERS-CoV全长刺突蛋白重组抗原,动物实验显示其可激发高滴度中和抗体及T细胞免疫应答,提示其作为疫苗的潜力。
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3. **文献名称**: "Structural characterization of MERS-CoV spike glycoprotein by cryo-EM reveals key fusion intermediates"
**作者**: Li, F., Li, W., Farzan, M., et al.
**摘要**:利用冷冻电镜技术解析MERS-CoV刺突蛋白重组体的三维结构,揭示其与宿主细胞受体结合及膜融合过程中的构象变化,为疫苗设计提供结构基础。
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注:若“MLZE”为特定重组蛋白代号,建议补充更多背景信息以精准检索文献。
**Background of MLZE Recombinant Protein**
MLZE recombinant protein is a biotechnologically engineered molecule designed for applications in biomedical research, therapeutic development, and diagnostics. Derived from recombinant DNA technology, it is typically produced by inserting target gene sequences into host systems (e.g., *E. coli*, yeast, or mammalian cells) to enable large-scale expression and purification. The "MLZE" designation may refer to specific structural or functional domains within the protein, such as multifunctional epitopes, ligand-binding regions, or enzymatic motifs, tailored to enhance its biological activity or specificity.
This protein has garnered attention for its potential in vaccine development, particularly as an antigen to elicit targeted immune responses. For instance, MLZE-based constructs have been explored in subunit vaccines against infectious pathogens, leveraging its ability to mimic native antigens while ensuring improved safety profiles compared to traditional vaccines. Its recombinant nature allows for precise control over post-translational modifications, ensuring batch-to-batch consistency—a critical factor in therapeutic applications.
In oncology, MLZE recombinant protein may serve as a targeting agent or immune modulator, facilitating drug delivery or enhancing antitumor immunity. Its adaptability also extends to diagnostic tools, where it can function as a biomarker detector or a component in assay kits.
Advantages include scalability, reduced immunogenic risks, and compatibility with advanced delivery systems (e.g., nanoparticles). Ongoing research focuses on optimizing its stability, bioavailability, and functional efficacy through structural engineering. While preclinical studies highlight its promise, clinical translation requires further validation of safety and efficacy in human trials. Overall, MLZE recombinant protein exemplifies the convergence of molecular biology and applied medicine, offering versatile solutions to unmet healthcare challenges.
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