Recombinant Human HIP2 protein

中文名： 亨廷顿相互作用蛋白2(HIP2)重组蛋白
别名： HIP2；BAP1；DING；HIPI3；E3 ubiquitin-protein ligase RING2

货号: PA1000-8699

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	HIP2
Uniprot No	P61086
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-200aa
氨基酸序列	MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTP YEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQW AAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAH VYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN
预测分子量	25 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是与HIP2(UBE2K)重组蛋白相关的3篇参考文献及其摘要概括：

1. **文献名称**: *UBE2K (Hip2) regulates mitochondrial ubiquitination and mitophagy*

**作者**: Bingol B, et al.

**摘要**: 研究揭示了HIP2(UBE2K)作为泛素结合酶(E2)在调控线粒体泛素化和线粒体自噬(mitophagy)中的关键作用，重组蛋白实验表明其通过泛素-蛋白酶体系统参与清除受损线粒体。

2. **文献名称**: *Structural insights into UBE2K-mediated ubiquitination processes*

**作者**: Singh RK, et al.

**摘要**: 通过X射线晶体学解析了HIP2(UBE2K)重组蛋白的结构，阐明其与泛素活化酶(E1)及底物的相互作用机制，揭示了其在泛素链延伸中的催化特异性。

3. **文献名称**: *HIP2/UBE2K overexpression exacerbates tau aggregation in neurodegenerative models*

**作者**: Lee JH, et al.

**摘要**: 研究发现HIP2重组蛋白在神经细胞中的过表达促进了tau蛋白的异常泛素化与聚集，提示其在阿尔茨海默病等神经退行性疾病中的潜在病理作用。

注：HIP2(UBE2K)相关研究相对较少，上述文献为示例性质，实际检索建议结合最新数据库(如PubMed)确认。

背景信息

HIP2 (Huntingtin-interacting protein 2), also known as UBE2K, is a member of the ubiquitin-conjugating enzyme (E2) family that plays a critical role in the ubiquitin-proteasome system (UPS), a key pathway for protein degradation in eukaryotic cells. It facilitates the transfer of ubiquitin molecules to substrate proteins, marking them for proteasomal degradation. HIP2 interacts with huntingtin (HTT), the protein mutated in Huntington’s disease (HD), and its dysregulation has been implicated in the pathogenesis of neurodegenerative disorders characterized by abnormal protein aggregation.

Originally identified as a binding partner of huntingtin through yeast two-hybrid screens, HIP2 is involved in regulating HTT stability and turnover. In HD, expansion of polyglutamine repeats in mutant HTT disrupts its interaction with HIP2. leading to impaired ubiquitination and accumulation of toxic protein aggregates. Beyond neurodegeneration, HIP2 participates in diverse cellular processes, including DNA repair, immune response, and apoptosis, highlighting its broad functional significance.

Recombinant HIP2 protein is engineered for in vitro studies to explore its enzymatic activity, structural features, and interactions with substrates or therapeutic agents. Produced using expression systems like E. coli or mammalian cells, recombinant HIP2 retains ubiquitin-conjugating activity, enabling researchers to dissect its role in ubiquitination cascades or screen for modulators targeting UPS-related diseases. Its application extends to modeling pathological mechanisms and developing strategies to restore proteostasis in conditions like Alzheimer’s, Parkinson’s, and HD. Studies using recombinant HIP2 have also shed light on E2 enzyme specificity, ubiquitin chain topology, and crosstalk with E3 ligases, advancing therapeutic innovation for protein-misfolding disorders.