| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HDC |
| Uniprot No | P19113 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-662aa |
| 氨基酸序列 | MMEPEEYRERGREMVDYICQYLSTVRERRVTPDVQPGYLRAQLPESAPEDPDSWDSIFGDIERIIMPGVVHWQSPHMHAYYPALTSWPSLLGDMLADAINCLGFTWASSPACTELEMNVMDWLAKMLGLPEHFLHHHPSSQGGGVLQSTVSESTLIALLAARKNKILEMKTSEPDADESCLNARLVAYASDQAHSSVEKAGLISLVKMKFLPVDDNFSLRGEALQKAIEEDKQRGLVPVFVCATLGTTGVCAFDCLSELGPICAREGLWLHIDAAYAGTAFLCPEFRGFLKGIEYADSFTFNPSKWMMVHFDCTGFWVKDKYKLQQTFSVNPIYLRHANSGVATDFMHWQIPLSRRFRSVKLWFVIRSFGVKNLQAHVRHGTEMAKYFESLVRNDPSFEIPAKRHLGLVVFRLKGPNCLTENVLKEIAKAGRLFLIPATIQDKLIIRFTVTSQFTTRDDILRDWNLIRDAATLILSQHCTSQPSPRVGNLISQIRGARAWACGTSLQSVSGAGDDPVQARKIIKQPQRVGAGPMKRENGLHLETLLDPVDDCFSEEAPDATKHKLSSFLFSYLSVQTKKKTVRSLSCNSVPVSAQKPLPTEASVKNGGSSRVRIFSRFPEDMMMLKKSAFKKLIKFYSVPSFPECSSQCGLQLPCCPLQAMV |
| 预测分子量 | 76.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HDC(组氨酸脱羧酶)重组蛋白的参考文献示例，基于学术文献的典型研究方向整理：
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1. **文献名称**：*Expression and Purification of Recombinant Histidine Decarboxylase from Lactobacillus parabuchneri in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达了来源于副布氏乳杆菌的HDC重组蛋白，通过优化表达条件和亲和层析技术实现了高效纯化，为后续酶活性和抑制剂研究奠定了基础。
2. **文献名称**：*Structural Insights into Human Histidine Decarboxylase: Implications for Drug Design*
**作者**：Zhang Y, et al.
**摘要**：利用X射线晶体学解析了人源HDC重组蛋白的三维结构，揭示了其催化活性中心的关键氨基酸残基，为开发针对组胺相关疾病(如过敏和胃癌)的抑制剂提供了结构基础。
3. **文献名称**：*Functional Characterization of Recombinant HDC in the Biosynthesis of Histamine*
**作者**：Lee J, et al.
**摘要**：通过酵母表达系统制备重组HDC蛋白，分析了其酶动力学参数及pH/温度稳定性，证实其在体外高效催化组胺合成的潜力，可用于工业化组胺生产。
4. **文献名称**：*Role of Recombinant HDC in Immune Regulation: Evidence from In Vitro Studies*
**作者**：Garcia R, et al.
**摘要**：研究发现，重组HDC蛋白通过调控免疫细胞中组胺的生成，影响Th2型免疫应答，提示其在过敏性疾病和炎症反应中的潜在治疗靶点。
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注：以上文献为示例，实际引用时建议通过PubMed、Web of Science等数据库检索最新或具体研究方向的论文，并核实作者、标题及摘要内容。
**Background of HDC Recombinant Protein**
Histidine decarboxylase (HDC) is a key enzyme responsible for catalyzing the conversion of L-histidine to histamine, a biogenic amine involved in numerous physiological and pathological processes. Histamine plays critical roles in immune responses, gastric acid secretion, neurotransmission, and allergic reactions. The HDC gene, located on human chromosome 15. encodes this enzyme, which is primarily expressed in mast cells, basophils, and enterochromaffin-like cells. Dysregulation of HDC activity or histamine levels has been linked to conditions such as allergies, asthma, peptic ulcers, and certain neurological disorders.
Recombinant HDC protein is produced using genetic engineering techniques, where the HDC gene is cloned into expression vectors and expressed in heterologous systems like *E. coli*, yeast, or mammalian cell lines. This approach enables large-scale production of purified, bioactive HDC for research and therapeutic applications. Recombinant HDC retains enzymatic activity, allowing scientists to study its structure-function relationships, regulatory mechanisms, and interactions with inhibitors or cofactors. It is also utilized in drug discovery to screen compounds targeting histamine biosynthesis, particularly for treating hyperacidity disorders or allergic diseases.
Additionally, recombinant HDC serves as a vital tool in diagnostics, aiding in the detection of histamine-related biomarkers or autoantibodies in clinical samples. Advances in protein engineering have further improved its stability and activity, facilitating its use in biotechnological and industrial settings. By providing a controlled and scalable source of HDC, recombinant technology continues to enhance our understanding of histamine biology and its therapeutic modulation.
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