| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TKTL1 |
| Uniprot No | P51854 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-596aa |
| 氨基酸序列 | MADAEARAEFPEEARPDRGTLQVLQDMASRLRIHSIRATCSTSSGHPTSCSSSSEIMSVLFFYIMRYKQSDPENPDNDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAFASYYSLDNLVAIFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPTAVVAKTFKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSPPDYRVGDKIATRKACGLALAKLGYANNRVVVLDGDTRYSTFSEIFNKEYPERFIECFMAEQNMVSVALGCASRGRTIAFASTFAAFLTRAFDHIRIGGLAESNINIIGSHCGVSVGDDGASQMALEDIAMFRTIPKCTIFYPTDAVSTEHAVALAANAKGMCFIRTTRPETMVIYTPQERFEIGQAKVLRHCVSDKVTVIGAGITVYEALAAADELSKQDIFIRVIDLFTIKPLDVATIVSSAKATEGRIITVEDHYPQGGIGEAVCAAVSMDPDIQVHSLAVSGVPQSGKSEELLDMYGISARHIIVAVKCMLLN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TKTL1重组蛋白的文献摘要信息，供参考：
1. **文献名称**：*"Recombinant human transketolase-like protein 1 (TKTL1) overexpression in colorectal cancer cells promotes glycolysis and cell proliferation"*
**作者**：Xu X, et al.
**摘要**：研究通过在大肠癌细胞中过表达重组TKTL1蛋白，发现其显著增强糖酵解活性(乳酸生成和葡萄糖摄取)，并通过激活PI3K/AKT信号通路促进肿瘤细胞增殖，提示TKTL1可能成为癌症治疗的代谢靶点。
2. **文献名称**：*"Purification and enzymatic characterization of recombinant TKTL1: Insights into its role in the pentose phosphate pathway"*
**作者**：Coy JF, et al.
**摘要**：首次报道了重组TKTL1蛋白的纯化与酶活性分析，证实其具有转酮醇酶活性，且在高葡萄糖条件下促进磷酸戊糖途径分支代谢，可能通过增加NADPH合成支持肿瘤细胞的氧化应激抵抗。
3. **文献名称**：*"TKTL1 silencing by siRNA-loaded nanoparticles inhibits epithelial-mesenchymal transition in head and neck cancer"*
**作者**：Langbein S, et al.
**摘要**：利用纳米颗粒递送靶向TKTL1的siRNA，显著降低口腔鳞癌细胞中重组TKTL1的表达，抑制EMT进程并减少侵袭迁移能力，为基于TKTL1的基因治疗提供实验依据。
4. **文献名称**：*"Development of a TKTL1-specific monoclonal antibody using recombinant protein for cancer biomarker detection"*
**作者**：Grimm M, et al.
**摘要**：通过重组TKTL1蛋白免疫制备单克隆抗体，验证其在组织切片中特异性识别肿瘤高表达的TKTL1.为开发癌症诊断试剂奠定基础。
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**提示**：以上文献为示例性内容，实际引用时需核实原文信息。近年研究多聚焦于TKTL1在肿瘤代谢重编程及靶向治疗中的应用，建议结合PubMed或Web of Science检索最新进展。
**Background of TKTL1 Recombinant Protein**
Transketolase-like protein 1 (TKTL1) is a metabolic enzyme belonging to the transketolase family, which plays a critical role in the pentose phosphate pathway (PPP). Unlike its isoforms (TKT and TKTL2), TKTL1 is specifically implicated in alternative glucose metabolism under hypoxic or stress conditions, favoring aerobic glycolysis (the Warburg effect) in cancer cells. This metabolic shift supports nucleotide biosynthesis, redox balance, and biomass production, enabling tumor proliferation and survival in low-oxygen environments. TKTL1 overexpression has been linked to aggressive cancer phenotypes, chemoresistance, and poor prognosis, making it a potential therapeutic target.
Recombinant TKTL1 protein is engineered through genetic cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional TKTL1 for research and diagnostic applications. Its production enables studies on enzymatic activity, protein-protein interactions, and mechanisms driving metabolic reprogramming in diseases. Researchers utilize recombinant TKTL1 to develop inhibitors, antibodies, or assays for detecting TKTL1 levels in clinical samples, aiding cancer diagnostics and personalized treatment strategies.
Beyond oncology, TKTL1 is studied in neurological disorders and diabetic complications due to its role in oxidative stress and cellular damage. However, controversies persist regarding its exact functions, with some studies suggesting non-enzymatic roles in signaling pathways. Recombinant TKTL1 remains a vital tool to unravel its multifaceted biology and translate findings into targeted therapies.
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