Recombinant Human TCF20 protein

中文名： 转录因子20(TCF20)重组蛋白
别名： TCF20；KIAA0292；SPBP；Transcription factor 20

货号: PA1000-8620

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	TCF20
Uniprot No	Q9UGU0
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	全长
氨基酸序列	full
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TCF20重组蛋白的模拟参考文献示例(实际文献需通过学术数据库检索)：

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1. **文献名称**: *"Expression and Purification of Recombinant TCF20 for Transcriptional Regulation Studies"*

**作者**: Smith, J. et al.

**摘要**: 本研究描述了在大肠杆菌中高效表达和纯化TCF20重组蛋白的方法，通过His标签亲和层析获得高纯度蛋白。实验验证了重组TCF20的DNA结合活性，并揭示了其与靶基因启动子区域的相互作用。

2. **文献名称**: *"Structural Insights into TCF20-DNA Complex by Cryo-EM"*

**作者**: Lee, H. & Zhang, Y.

**摘要**: 利用冷冻电镜技术解析了TCF20重组蛋白与DNA复合物的三维结构，揭示了其锌指结构域的关键结合位点，为理解TCF20在转录调控中的分子机制提供了结构基础。

3. **文献名称**: *"Functional Characterization of TCF20 Isoforms in Cell Differentiation"*

**作者**: García-Ruiz, C. et al.

**摘要**: 通过体外重组蛋白实验和细胞模型，发现TCF20的不同剪接变体对干细胞分化的调控功能存在差异，其中重组TCF20-α变体显著促进神经元特异性基因的表达。

4. **文献名称**: *"TCF20 Interactome Analysis via Recombinant Protein Pull-Down Assays"*

**作者**: Patel, R. & Kim, S.

**摘要**: 采用重组TCF20蛋白进行pull-down联合质谱分析，鉴定了包括染色质重塑复合物成员在内的多个相互作用蛋白，提示TCF20可能通过多蛋白复合物参与表观遗传调控。

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**备注**：以上文献为模拟示例，实际研究需通过**PubMed/Google Scholar**等平台检索(可尝试关键词：`TCF20 recombinant protein`、`TCF20 structure/function`)。如需具体文献协助，请提供更多研究背景信息。

背景信息

**Background of TCF20 Recombinant Protein**

TCF20 (Transcription Factor 20), also known as AR1 or SPIN, is a multifunctional nuclear protein belonging to the transcription factor family. It plays critical roles in regulating gene expression, chromatin remodeling, and cellular signaling pathways. Structurally, TCF20 contains several conserved domains, including an N-terminal activation domain (AD), a central DNA-binding domain (DBD), and a C-terminal proline-rich domain (PRD), which facilitate interactions with other proteins and DNA.

TCF20 is implicated in diverse biological processes, such as neurodevelopment, cell proliferation, and differentiation. Studies highlight its involvement in neural circuit formation and synaptic plasticity, linking it to neurodevelopmental disorders like autism spectrum disorder (ASD) and schizophrenia. Additionally, TCF20 interacts with tumor suppressors (e.g., p53) and oncoproteins, modulating pathways related to apoptosis and genome stability. Dysregulation of TCF20 has been observed in cancers, including breast and lung cancers, where it may act as either a tumor suppressor or oncogene depending on cellular context.

Recombinant TCF20 protein is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag). This engineered protein retains functional domains, enabling in vitro studies to dissect its DNA-binding properties, protein-protein interactions, and post-translational modifications (e.g., phosphorylation). Researchers utilize recombinant TCF20 to investigate its role in transcriptional regulation, screen for small-molecule modulators, or develop diagnostic tools for associated diseases.

Overall, TCF20 recombinant protein serves as a vital tool for unraveling its molecular mechanisms and exploring therapeutic strategies for neurodevelopmental disorders and cancers.