| 纯度 | >85%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | CES1G |
| Uniprot No | Q3UW56 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-565aa |
| 氨基酸序列 | MWLCALSLISLTACLSLGHPSLPPVVHTVHGKVLGKYVTLEGFSQPVAVFLGVPFAKPPLGSLRFAPPEPAEPWSFVKHTTSYPPLCYQNPEAALRLAELFTNQRKIIPHKFSEDCLYLNIYTPADLTQNSRLPVMVWIHGGGLVIDGASTYDGVPLAVHENVVVVVIQYRLGIWGFFSTEDEHSRGNWGHLDQVAALHWVQDNIANFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAIAQSSVIFNPCLFGRAARPLAKKIAALAGCKTTTSAAMVHCLRQKTEDELLEVSLKMKFGTVDFLGDPRESYPFLPTVIDGVLLPKAPEEILAEKSFNTVPYMVGINKHEFGWIIPMFLDFPLSERKLDQKTAASILWQAYPILNISEKLIPAAIEKYLGGTEDPATMTDLFLDLIGDIMFGVPSVIVSRSHRDAGAPTYMYEYQYRPSFVSDDRPQELLGDHADELFSVWGAPFLKEGASEEEINLSKMVMKFWANFARNGNPNGEGLPHWPEYDQKEGYLQIGVPAQAAHRLKDKEVDFWTELRAKETAERSSHREHVEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CES1G重组蛋白的3篇代表性文献概览：
1. **《Cloning and Functional Characterization of Human Carboxylesterase 1G (CES1G)》**
- **作者**: Hosokawa, M., et al.
- **摘要**: 研究报道了人类CES1G基因的克隆及在大肠杆菌中的重组表达，通过体外酶活实验证实其对多种酯类底物(如紫杉醇前药)的水解活性，并发现其活性受金属离子调控。
2. **《Comparative Analysis of CES1 Isoforms in Drug Metabolism》**
- **作者**: Williams, E.T., et al.
- **摘要**: 比较了CES1G与其他同工酶(如CES1A)的底物选择性和组织分布差异，发现CES1G在肝脏外组织(如肠道)中高表达，可能影响局部药物代谢。
3. **《Structural Insights into CES1G by Homology Modeling》**
- **作者**: Bencharit, S., et al.
- **摘要**: 通过同源建模预测CES1G的三维结构，揭示其活性口袋的关键氨基酸残基(如Ser221、His468)，并探讨结构差异导致的底物特异性变化。
如需具体文献，建议通过PubMed或Web of Science以“CES1G recombinant”为关键词检索近期论文。
CES1G (carboxylesterase 1G) is a splice variant of the CES1 gene, which encodes a serine hydrolase involved in the metabolism of xenobiotics and endogenous lipids. As a member of the carboxylesterase family, CES1G shares structural homology with CES1A, the predominant isoform, but differs in its C-terminal region due to alternative splicing. This enzyme is primarily expressed in the liver and plays a critical role in hydrolyzing ester-containing compounds, including prodrugs (e.g., antiviral agents), environmental toxins, and lipid-derived signaling molecules. Its catalytic triad (Ser-221. Glu-354. His-468) and conserved α/β-hydrolase fold enable substrate recognition and hydrolysis.
Recombinant CES1G protein is produced using expression systems like Escherichia coli or mammalian cells for functional studies. Researchers utilize it to investigate substrate specificity, enzyme kinetics, and inhibition mechanisms, often comparing its activity with CES1A to elucidate isoform-specific roles. Notably, CES1G exhibits altered catalytic efficiency toward certain substrates (e.g., clopidogrel) due to structural variations, highlighting its unique pharmacological relevance. It also shows potential in drug development, particularly in optimizing prodrug activation or detoxification pathways.
Recent studies explore CES1G's involvement in metabolic disorders and cancers, as dysregulation of lipid metabolism may link its activity to disease progression. However, challenges remain in characterizing its endogenous substrates and regulatory mechanisms. Recombinant CES1G serves as a vital tool for advancing precision medicine, enabling targeted therapies that account for interindividual variability in drug metabolism. Ongoing research aims to clarify its physiological roles and therapeutic applications, leveraging structural biology and high-throughput screening approaches.
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