| 纯度 | >90%SDS-PAGE. |
| 种属 | E. coli |
| 靶点 | TKL1 |
| Uniprot No | Q12630 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-679aa |
| 氨基酸序列 | MSQYTDIDRLAVSTIRLLAVDQVSAANSGHPGAPLGLAPAAHVIWKQMRLNPKNPEWINRDRFVLSNGHACALLYSLLHLFGYDMSIEDLKHFRHLGSKTPGHPEFELPGVEVTTGPLGQGISNAVGMAIAQANFAATYNKPDYELSDSYTYVFLGDGCLQEGVSSEASSLAGHLRLKNLIAFYDDNQITIDGNINVSFDEDVSKRYEAYGWEVLHVENGNDDLDAISKALEQAKLSDRPTLIKLTTTIGFGSLNAGSHSVHGAPLKADDVKQLKVKFGFNPEESFVVPQEVYDLYNKSTIEPGIEANKQWDALLDAYVGQFPELGAEVKRRLAGEFPEGWESKLPTYTPEDSAVASRKLSEIVLDNVFDTLPELLGGSADLTPSNLTRSKGAVDFQPPITGLGDYSGRYIRYGVREHGMGAIMNGISAFGANYRPYGGTFLNFVSYASGAVRLSALSGHPVIWVATHDSIGLGEDGPTHQPIETLAHFRAIPNLQVWRPADGNEVTAAYKVALTNKHTPAIIALSRQNLPQLQGSSVEKAVKGGYILQDVDQPDLAIVSTGSEVGIAVEAAKVLAEKNIKARIVSLPDFHSFGQQSKEYQLSVFPDGVPILSVEVLATSGWSKYAHQSFGLDRFGASGKGPAVYEKFEFTPQGIATRAEKTVEFYKGKQVISPLNTAF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TKL1重组蛋白的3篇参考文献示例(文献信息为模拟内容，供参考)：
1. **文献名称**: "Cloning and Expression of Recombinant TKL1 in E. coli for Functional Analysis"
**作者**: Smith J, et al.
**摘要**: 研究报道了人源TKL1基因的克隆及在大肠杆菌中的重组表达，通过亲和层析纯化获得高纯度蛋白，并验证其激酶活性，为后续功能研究提供基础。
2. **文献名称**: "Structural Characterization of TKL1 Recombinant Protein Using X-ray Crystallography"
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析TKL1重组蛋白的三维结构，揭示其激酶结构域的关键功能位点，为设计靶向抑制剂提供结构依据。
3. **文献名称**: "TKL1 Recombinant Protein Modulates Immune Response in Macrophages"
**作者**: Zhang Y, et al.
**摘要**: 研究利用昆虫细胞表达系统制备TKL1重组蛋白，发现其通过调控MAPK信号通路影响巨噬细胞的炎症因子分泌，提示其在免疫调节中的潜在作用。
注：以上文献为示例，实际研究中建议通过PubMed或Web of Science等数据库检索具体文献。若需真实文献，可补充关键词或研究背景进一步筛选。
TKL1 (Tyrosine Kinase-Like 1) recombinant protein is a engineered form of the TKL1 protein, which belongs to the tyrosine kinase-like (TKL) subgroup within the serine/threonine/tyrosine kinase superfamily. TKL1 shares structural homology with tyrosine kinases but primarily functions as a serine/threonine kinase, playing regulatory roles in cellular signaling pathways. It is implicated in processes such as cell proliferation, differentiation, and stress responses, though its exact physiological substrates and mechanisms remain under investigation. The gene encoding TKL1 is evolutionarily conserved, with orthologs identified in mammals, plants, and fungi, suggesting broad functional significance.
Recombinant TKL1 is typically produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable large-scale purification for research applications. The protein is often tagged with affinity markers (e.g., His-tag, GST) to facilitate isolation and detection. Its recombinant form retains kinase activity, making it valuable for in vitro studies of enzymatic kinetics, substrate identification, and inhibitor screening. Researchers utilize TKL1 recombinant protein to explore its involvement in disease pathways, particularly cancer and inflammatory disorders, where aberrant kinase activity is frequently observed. Additionally, it serves as a tool for developing targeted therapies, as dysregulated TKL1 signaling has been linked to tumor progression and immune dysregulation.
Current studies focus on characterizing TKL1's interaction networks and post-translational modifications, aiming to elucidate its role in cellular homeostasis. The availability of recombinant TKL1 accelerates structural studies (e.g., crystallography) and high-throughput drug discovery, bridging gaps between basic research and therapeutic innovation.
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