| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSC22 |
| Uniprot No | O75157 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-780aa |
| 氨基酸序列 | MSKMPAKKKS CFQITSVTTA QVATSITEDT ESLDDPDESR TEDVSSEIFD VSRATDYGPE EVCERSSSEE TLNNVGDAET PGTVSPNLLL DGQLAAAAAA PANGGGVVSA RSVSGALAST LAAAATSAPA PGAPGGPQLA GSSAGPVTAA PSQPPTTCSS RFRVIKLDHG SGEPYRRGRW TCMEYYERDS DSSVLTRSGD CIRHSSTFDQ TAERDSGLGA TGGSVVVVVA SMQGAHGPES GTDSSLTAVS QLPPSEKMSQ PTPAQPQSFS VGQPQPPPPP VGGAVAQSSA PLPPFPGAAT GPQPMMAAAQ PSQPQGAGPG GQTLPPTNVT LAQPAMSLPP QPGPAVGAPA AQQPQQFAYP QPQIPPGHLL PVQPSGQSEY LQQHVAGLQP PSPAQPSSTG AAASPATAAT LPVGTGQNAS SVGAQLMGAS SQPSEAMAPR TGPAQGGQVA PCQPTGVPPA TVGGVVQPCL GPAGAGQPQS VPPPQMGGSG PLSAVPGGPH AVVPGVPNVP AAVPAPSVPS VSTTSVTMPN VPAPLAQSQQ LSSHTPVSRS SSIIQHVGLP LAPGTHSAPT SLPQSDLSQF QTQTQPLVGQ VDDTRRKSEP LPQPPLSLIA ENKPVVKPPV ADSLANPLQL TPMNSLATSV FSIAIPVDGD EDRNPSTAFY QAFHLNTLKE SKSLWDSASG GGVVAIDNKI EQAMDLVKSH LMYAVREEVE VLKEQIKELV ERNSLLEREN ALLKSLSSND QLSQLPTQQA NPGSTSQQQA VIAQPPQPTQ PPQQPNVSSA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TSC22重组蛋白的参考文献概览(文献标题及摘要内容为虚构示例，仅供格式参考)：
1. **标题**: *"Functional characterization of recombinant TSC22D1 protein in TGF-β signaling regulation*"
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌表达系统重组表达TSC22D1蛋白，证实其通过结合Smad蛋白抑制TGF-β信号通路，调控细胞增殖与凋亡。
2. **标题**: *"Expression and purification of TSC22D2 recombinant protein for structural analysis*"
**作者**: Lee JH, et al.
**摘要**: 利用昆虫细胞系统成功表达并纯化TSC22D2重组蛋白，通过X射线晶体学解析其三维结构，揭示其转录调控功能的关键结构域。
3. **标题**: *"TSC22 recombinant protein attenuates renal fibrosis by modulating autophagy*"
**作者**: Zhang Y, et al.
**摘要**: 体外实验表明，重组TSC22蛋白通过激活自噬通路抑制肾小管上皮细胞纤维化，为慢性肾病治疗提供潜在靶点。
(注：以上内容为模拟生成，实际文献需通过PubMed或Google Scholar检索关键词“TSC22 recombinant protein”获取。)
**Background of TSC22 Recombinant Protein**
The TSC22 (TGF-β-stimulated clone 22) family of proteins, particularly TSC22D1. is a conserved group of regulatory molecules implicated in diverse cellular processes, including cell proliferation, differentiation, apoptosis, and stress responses. First identified as a transcript upregulated by TGF-β signaling in murine osteoblasts, TSC22D1 has since been recognized for its role as a transcriptional co-regulator or adaptor protein, interacting with pathways such as TGF-β/Smad, PI3K/AKT/mTOR, and glucocorticoid signaling. Structurally, TSC22D1 contains a TSC22 domain and leucine zipper motifs, which facilitate protein-protein interactions and dimerization.
Recombinant TSC22 proteins are engineered to study its biological functions or therapeutic potential. These proteins are typically produced in expression systems like *E. coli* or mammalian cells, ensuring proper folding and post-translational modifications. Research highlights TSC22D1's dual roles in disease contexts: it acts as a tumor suppressor in certain cancers (e.g., inhibiting prostate cancer progression) but may promote fibrosis or inflammation in conditions like renal fibrosis or osteoarthritis. Its ability to modulate gene expression and cellular stress responses has also linked it to metabolic disorders and neurodegenerative diseases.
The development of TSC22 recombinant proteins aids in elucidating molecular mechanisms, screening drug candidates, or exploring biomarkers. Challenges remain in understanding context-dependent interactions and tissue-specific isoforms. Nonetheless, TSC22 recombinant tools continue to advance research in cancer biology, fibrosis, and immune regulation, offering potential for targeted therapies.
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