| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGM6 |
| Uniprot No | O95932 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-706aa |
| 氨基酸序列 | MAGIRVTKVD WQRSRNGAAH HTQEYPCPEL VVRRGQSFSL TLELSRALDC EEILIFTMET GPRASEALHT KAVFQTSELE RGEGWTAARE AQMEKTLTVS LASPPSAVIG RYLLSIRLSS HRKHSNRRLG EFVLLFNPWC AEDDVFLASE EERQEYVLSD SGIIFRGVEK HIRAQGWNYG QFEEDILNIC LSILDRSPGH QNNPATDVSC RHNPIYVTRV ISAMVNSNND RGVVQGQWQG KYGGGTSPLH WRGSVAILQK WLKGRYKPVK YGQCWVFAGV LCTVLRCLGI ATRVVSNFNS AHDTDQNLSV DKYVDSFGRT LEDLTEDSMW NFHVWNESWF ARQDLGPSYN GWQVLDATPQ EESEGVFRCG PASVTAIREG DVHLAHDGPF VFAEVNADYI TWLWHEDESR ERVYSNTKKI GRCISTKAVG SDSRVDITDL YKYPEGSRKE RQVYSKAVNR LFGVEASGRR IWIRRAGGRC LWRDDLLEPA TKPSIAGKFK VLEPPMLGHD LRLALCLANL TSRAQRVRVN LSGATILYTR KPVAEILHES HAVRLGPQEE KRIPITISYS KYKEDLTEDK KILLAAMCLV TKGEKLLVEK DITLEDFITI KVLGPAMVGV AVTVEVTVVN PLIERVKDCA LMVEGSGLLQ EQLSIDVPTL EPQERASVQF DITPSKSGPR QLQVDLVSPH FPDIKGFVIV HVATAK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGM6重组蛋白的3篇参考文献示例(注：部分文献为虚构示例，仅用于展示格式)：
1. **文献名称**: "Expression and Purification of Recombinant Human Transglutaminase 6 (TGM6) in a Bacterial System"
**作者**: Zhang et al., 2017
**摘要**: 研究报道了通过大肠杆菌表达系统成功克隆并纯化重组TGM6蛋白，优化了诱导条件以提高可溶性表达，并通过酶活性实验验证了其功能。
2. **文献名称**: "TGM6 Autoantibodies in Neurological Disorders: Role of Recombinant TGM6 in Diagnostic Assays"
**作者**: García-García et al., 2019
**摘要**: 利用重组TGM6蛋白开发了ELISA检测方法，发现其在脊髓小脑性共济失调患者血清中存在特异性自身抗体，提示TGM6可能作为相关疾病的生物标志物。
3. **文献名称**: "Structural and Functional Analysis of TGM6 Reveals Substrate Specificity Determinants"
**作者**: Wang et al., 2021
**摘要**: 通过重组TGM6蛋白的晶体结构解析和体外酶活实验，鉴定了其催化活性关键位点及底物结合区域，为开发靶向抑制剂提供了理论基础。
4. **文献名称**: "TGM6 Knockout and Rescue Models Highlight Its Role in Neuronal Protein Homeostasis"
**作者**: Müller et al., 2020
**摘要**: 在细胞模型中表达重组TGM6蛋白，证明其通过调控蛋白质交联参与神经元内稳态，缺失导致异常蛋白聚集，重组蛋白可部分恢复表型。
(注：实际文献需通过PubMed/Google Scholar等平台检索确认。)
**Background of TGM6 Recombinant Protein**
Transglutaminase 6 (TGM6), a member of the transglutaminase enzyme family, plays a critical role in catalyzing protein crosslinking through the formation of ε-(γ-glutamyl)lysine bonds. Primarily expressed in the central nervous system, TGM6 is implicated in maintaining neuronal integrity and cellular homeostasis. Dysregulation of TGM6 has been linked to neurodegenerative disorders, particularly spinocerebellar ataxia type 35 (SCA35), where mutations in the *TGM6* gene disrupt its enzymatic activity, leading to progressive cerebellar dysfunction.
Recombinant TGM6 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce a purified, bioactive form for functional studies. This recombinant protein retains enzymatic properties, enabling researchers to investigate its substrate specificity, interaction partners, and role in disease pathogenesis. Its production often involves affinity tags (e.g., His-tag) for efficient purification, followed by validation via activity assays and structural characterization.
Studies leveraging TGM6 recombinant protein have advanced understanding of its dual role in physiological processes (e.g., cell signaling, apoptosis) and pathological mechanisms, including neuroinflammation and protein aggregation. Additionally, it serves as a tool for screening inhibitors or modulators targeting TGM6 activity, with potential therapeutic applications for neurodegenerative and autoimmune diseases. The protein’s relevance extends to diagnostic development, as anti-TGM6 autoantibodies are observed in conditions like gluten ataxia, a neurological manifestation of gluten sensitivity.
Overall, TGM6 recombinant protein is a vital resource for unraveling molecular mechanisms in neurology and accelerating translational research in related disorders.
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