| 纯度 | >85%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Ces1d |
| Uniprot No | Q8VCT4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-565aa |
| 氨基酸序列 | YP SSPPVVNTVK GKVLGKYVNL EGFTQPVAVF LGVPFAKPPL GSLRFAPPQP AEPWSFVKNT TSYPPMCSQD AVGGQVLSEL FTNRKENIPL QFSEDCLYLN IYTPADLTKN SRLPVMVWIH GGGLVVGGAS TYDGLALSAH ENVVVVTIQY RLGIWGFFST GDEHSRGNWG HLDQVAALRW VQDNIANFGG NPGSVTIFGE SAGGFSVSVL VLSPLAKNLF HRAISESGVS LTAALITTDV KPIAGLVATL SGCKTTTSAV MVHCLRQKTE DELLETSLKL NLFKLDLLGN PKESYPFLPT VIDGVVLPKA PEEILAEKSF STVPYIVGIN KQEFGWIIPT LMGYPLAEGK LDQKTANSLL WKSYPTLKIS ENMIPVVAEK YLGGTDDLTK KKDLFQDLMA DVVFGVPSVI VSRSHRDAGA STYMYEFEYR PSFVSAMRPK AVIGDHGDEI FSVFGSPFLK DGASEEETNL SKMVMKFWAN FARNGNPNGG GLPHWPEYDQ KEGYLKIGAS TQAAQRLKDK EVSFWAELRA KESAQRPSHR EHVEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **Ces1d重组蛋白** 的3篇模拟参考文献示例(实际文献需通过学术数据库检索)：
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1. **文献名称**：*"Expression and Characterization of Recombinant CES1D in a Bacterial System for Substrate Specificity Analysis"*
**作者**：Wang Y. et al.
**摘要**：研究在大肠杆菌中高效表达并纯化重组Ces1d蛋白，优化表达条件后，通过体外酶活实验验证其对多种酯类底物(如药物前体及脂质类似物)的水解活性，发现其对长链脂肪酸酯具有偏好性。
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2. **文献名称**：*"Structural Insights into Human CES1D Enzyme by X-ray Crystallography and Mutagenesis"*
**作者**：Li H. et al.
**摘要**：解析了Ces1d重组蛋白的晶体结构，揭示其催化三联体(Ser-His-Glu)及底物结合口袋的构象特征，并通过点突变实验证明特定氨基酸残基对酶活性和底物选择性的关键作用。
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3. **文献名称**：*"Role of Recombinant CES1D in Lipid Metabolism: Implications for Hepatic Steatosis"*
**作者**：Kim S. et al.
**摘要**：利用重组Ces1d蛋白研究其在甘油三酯水解中的作用，发现其过表达可减少肝细胞脂质蓄积，提示Ces1d可能通过调节脂代谢通路参与非酒精性脂肪肝的病理过程。
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**注**：以上为模拟示例，实际文献需通过 **PubMed/Google Scholar** 等平台检索关键词(如 "CES1D recombinant protein"、"Carboxylesterase 1D expression")获取。
**Background of Ces1d Recombinant Protein**
Carboxylesterase 1D (Ces1d), also known as triglyceride hydrolase or Ces3. is a member of the carboxylesterase enzyme family, which plays critical roles in lipid metabolism, drug activation, and detoxification. In mammals, Ces1d is predominantly expressed in the liver and adipose tissue, where it hydrolyzes ester-containing substrates, including triglycerides, cholesterol esters, and xenobiotics. This serine-dependent hydrolase is encoded by the *Ces1d* gene and shares structural homology with other carboxylesterases, featuring a conserved α/β-hydrolase fold and a catalytic triad (Ser-His-Glu).
Research highlights Ces1d's importance in regulating systemic energy homeostasis. Studies in murine models show that Ces1d deficiency leads to altered lipid profiles, including elevated plasma triglycerides and reduced hepatic lipid storage, linking it to metabolic disorders such as obesity and insulin resistance. Ces1d also interacts with pharmacological agents, influencing the metabolism of ester prodrugs (e.g., angiotensin-converting enzyme inhibitors) and environmental toxins, underscoring its biomedical relevance.
Recombinant Ces1d protein is engineered for *in vitro* studies to dissect its enzymatic mechanisms, substrate specificity, and inhibitor screening. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the purified protein retains catalytic activity, enabling structural analysis (e.g., X-ray crystallography) and functional assays. Its applications extend to drug development, toxicology, and metabolic disease research, offering insights into lipid dysregulation pathways and therapeutic targeting.
Overall, Ces1d recombinant protein serves as a vital tool for exploring the enzyme's physiological roles and its potential as a biomarker or drug target in metabolic syndromes.
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