| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PER1 |
| Uniprot No | O15534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1290aa |
| 氨基酸序列 | MSGPLEGADGGGDPRPGESFCPGGVPSPGPPQHRPCPGPSLADDTDANSNGSSGNESNGHESRGASQRSSHSSSSGNGKDSALLETTESSKSTNSQSPSPPSSSIAYSLLSASSEQDNPSTSGCSSEQSARARTQKELMTALRELKLRLPPERRGKGRSGTLATLQYALACVKQVQANQEYYQQWSLEEGEPCSMDMSTYTLEELEHITSEYTLQNQDTFSVAVSFLTGRIVYISEQAAVLLRCKRDVFRGTRFSELLAPQDVGVFYGSTAPSRLPTWGTGASAGSGLRDFTQEKSVFCRIRGGPDRDPGPRYQPFRLTPYVTKIRVSDGAPAQPCCLLIAERIHSGYEAPRIPPDKRIFTTRHTPSCLFQDVDERAAPLLGYLPQDLLGAPVLLFLHPEDRPLMLAIHKKILQLAGQPFDHSPIRFCARNGEYVTMDTSWAGFVHPWSRKVAFVLGRHKVRTAPLNEDVFTPPAPSPAPSLDTDIQELSEQIHRLLLQPVHSPSPTGLCGVGAVTSPGPLHSPGSSSDSNGGDAEGPGPPAPVTFQQICKDVHLVKHQGQQLFIESRARPQSRPRLPATGTFKAKALPCQSPDPELEAGSAPVQAPLALVPEEAERKEASSCSYQQINCLDSILRYLESCNLPSTTKRKCASSSSYTTSSASDDDRQRTGPVSVGTKKDPPSAALSGEGATPRKEPVVGGTLSPLALANKAESVVSVTSQCSFSSTIVHVGDKKPPESDIIMMEDLPGLAPGPAPSPAPSPTVAPDPAPDAYRPVGLTKAVLSLHTQKEEQAFLSRFRDLGRLRGLDSSSTAPSALGERGCHHGPAPPSRRHHCRSKAKRSRHHQNPRAEAPCYVSHPSPVPPSTPWPTPPATTPFPAVVQPYPLPVFSPRGGPQPLPPAPTSVPPAAFPAPLVTPMVALVLPNYLFPTPSSYPYGALQTPAEGPPTPASHSPSPSLPALAPSPPHRPDSPLFNSRCSSPLQLNLLQLEELPRAEGAAVAGGPGSSAGPPPPSAEAAEPEARLAEVTESSNQDALSGSSDLLELLLQEDSRSGTGSAASGSLGSGLGSGSGSGSHEGGSTSASITRSSQSSHTSKYFGSIDSSEAEAGAARGGAEPGDQVIKYVLQDPIWLLMANADQRVMMTYQVPSRDMTSVLKQDRERLRAMQKQQPRFSEDQRRELGAVHSWVRKGQLPRALDVMACVDCGSSTQDPGHPDDPLFSELDGLGLEPMEEGGGEQGSSGGGSGEGEGCEEAQGGAKASSSQDLAMEEEEEGRSSSSPALPTAGNCTS |
| 预测分子量 | 136,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PER1重组蛋白的3篇参考文献示例(注：文献信息为示例性质，建议通过学术数据库查询最新真实文献)：
1. **《Recombinant PER1 protein inhibits tumor growth by modulating cell cycle in colorectal cancer》**
- 作者：Chen, L., et al.
- 摘要：本研究通过在大肠杆菌中表达并纯化人源PER1重组蛋白，发现其能通过调控Cyclin D1和p21表达，阻滞结直肠癌细胞G1/S期转换，抑制肿瘤增殖。
2. **《Expression and functional characterization of PER1 recombinant protein in circadian regulation》**
- 作者：Wang, Y., et al.
- 摘要：利用昆虫细胞系统表达PER1重组蛋白，结合体外激酶实验证实其与CK1ε的磷酸化相互作用，揭示了PER1在生物钟振荡中的核心调控机制。
3. **《Structural insights into PER1-CRY2 complex formation using recombinant proteins》**
- 作者：Kim, S., et al.
- 摘要：通过共表达并纯化PER1与CRY2重组蛋白，利用X射线晶体学解析复合物结构，阐明两者结合的关键结构域及对昼夜节律负反馈环路的影响。
建议通过PubMed或Google Scholar检索关键词“PER1 recombinant protein expression”、“PER1 functional study”等获取具体文献。
PER1 (Period Circadian Regulator 1) is a core component of the mammalian circadian clock system, playing a critical role in regulating daily physiological and behavioral rhythms. It belongs to the Period family of genes, which oscillate in expression under the control of transcriptional-translational feedback loops. The PER1 protein interacts with other clock components, such as CRY (Cryptochrome) proteins, to inhibit the activity of CLOCK-BMAL1 heterodimers, thereby repressing their own transcription. This negative feedback mechanism ensures ~24-hour rhythmicity in gene expression, coordinating processes like metabolism, sleep-wake cycles, and hormone secretion.
Recombinant PER1 protein is artificially produced using expression systems (e.g., E. coli, mammalian cells) for functional studies. Its production enables researchers to investigate post-translational modifications (e.g., phosphorylation by CK1ε/δ), protein-protein interactions, and degradation pathways involving ubiquitin-proteasome systems. Structural analysis of recombinant PER1 has revealed PAS domains essential for dimerization and nuclear localization signals guiding its subcellular trafficking.
Research on PER1 extends beyond circadian biology. Dysregulation of PER1 has been linked to cancer progression, sleep disorders, metabolic syndromes, and neurological conditions. For instance, aberrant PER1 expression correlates with tumor suppression in colorectal and breast cancers, while polymorphisms are associated with altered sleep timing. Recombinant PER1 also serves as a tool for drug screening, particularly for compounds targeting circadian rhythm disorders or chronotherapy optimization.
The protein's conserved evolutionary role underscores its importance in maintaining temporal homeostasis. Studying recombinant PER1 enhances understanding of circadian disruption mechanisms and potential therapeutic interventions, bridging molecular insights with clinical applications.
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