| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ntn1 |
| Uniprot No | O95631 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-604aa |
| 氨基酸序列 | GPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNASDPK KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA FSRLHTFGDE NEDDSELARD SYFYAVSDLQ VGGRCKCNGH AARCVRDRDD SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGYYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKINMKKY CKKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK CKKA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Ntn1(Netrin-1)重组蛋白的模拟参考文献示例，供参考：
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1. **文献名称**：重组Netrin-1蛋白在大肠杆菌中的高效表达与功能鉴定
**作者**：Zhang L, et al.
**摘要**：本研究通过基因工程技术在大肠杆菌中成功表达并纯化了具有生物活性的重组Netrin-1蛋白。实验表明，纯化的蛋白能够促进神经元轴突的体外生长，并通过受体DCC介导的信号通路发挥作用，为神经再生研究提供了工具。
2. **文献名称**：Netrin-1重组蛋白在胶质母细胞瘤迁移中的双重作用机制
**作者**：Smith J, et al.
**摘要**：文章利用哺乳动物细胞表达系统制备了重组Netrin-1.发现其可通过依赖浓度梯度的方式调控肿瘤细胞迁移。高浓度Netrin-1抑制胶质瘤细胞侵袭，而低浓度则具有趋化效应，揭示了其在癌症中的复杂功能。
3. **文献名称**：Netrin-1重组蛋白的结构解析及其与受体Unc5B的相互作用
**作者**：Lee S, et al.
**摘要**：通过X射线晶体学解析了重组Netrin-1的晶体结构，并证明其N端结构域是结合Unc5B受体的关键区域。该研究为设计靶向Netrin-1信号通路的药物提供了结构基础。
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**注意**：以上文献为示例性质，具体研究需通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。建议使用关键词“recombinant Netrin-1”或“Ntn1 protein expression”进行精准查询。
Netrin-1 (Ntn1), a secreted protein belonging to the Netrin family, plays critical roles in axonal guidance, cell migration, and tissue development during embryogenesis and adult homeostasis. It was initially identified for its ability to guide commissural axons across the midline in the nervous system via interactions with receptors such as DCC (Deleted in Colorectal Cancer) and Unc5. Structurally, Netrin-1 contains laminin-like domains and a C-terminal domain, enabling dual functionality as both a chemoattractant and chemorepellent depending on receptor context. Beyond neural development, Netrin-1 is implicated in angiogenesis, inflammation, and cancer progression, where it often exhibits paradoxical roles—promoting tumor vascularization or metastasis in some contexts while suppressing apoptosis in others.
Recombinant Netrin-1 protein, produced through mammalian or insect expression systems, retains bioactive properties for experimental and therapeutic applications. Its production typically involves codon-optimized cDNA transfection, affinity chromatography purification (e.g., His-tag), and validation via functional assays like neurite outgrowth in neuronal cultures. Researchers leverage recombinant Netrin-1 to study mechanisms of neural regeneration, cancer cell invasiveness, and endothelial cell behavior. Notably, dysregulated Netrin-1 signaling is linked to neurodegenerative diseases (e.g., Alzheimer’s) and chemotherapy resistance, spurring interest in targeting Netrin-1 pathways for drug development. However, its pleiotropic effects necessitate precise contextual evaluation in disease models. As a tool, recombinant Netrin-1 remains indispensable for dissecting extracellular signaling networks and exploring regenerative or anti-cancer strategies.
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