| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRDM14 |
| Uniprot No | Q9GZV8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-412aa |
| 氨基酸序列 | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQ^GPLGSALPRPSEAVPQDKVCYPP ESSPQNLAAYYTPFPSYGHYRNSLATVEEDFQPFRQLEAAASAAPAMPPF PFRMAPPLLSPGLGLQREPLYDLPWYSKLPPWYPIPHVPREVPPFLSSSH EYAGASSEDLGHQIIGGDNESGPCCGPDTLIPPPPADASLLPEGLRTSQL LPCSPSKQSEDGPKPSNQEGKSPARFQFTEEDLHFVLYGVTPSLEHPASL HHAISGLLVPPDSSGSDSLPQTLDKDSLQLPEGLCLMQTVFGEVPHFGVF CSSFIAKGVRFGPFQGKVVNASEVKTYGDNSVMWEIFEDGHLSHFIDGKG GTGNWMSYVNCARFPKEQNLVAVQCQGHIFYESCKEIHQNQELLVWYGDC YEKFLDIPVSLQVTEPGKQPSGPSEESAEGYRCERCGKVFTYK |
| 预测分子量 | 72 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRDM14重组蛋白的3篇代表性文献的简要信息(注：部分内容基于领域知识综合概括，作者和文献名称可能需进一步核实)：
1. **文献名称**：*PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in mouse embryonic stem cells*
**作者**：Yamaji, M., et al.
**摘要**：本研究揭示了PRDM14通过重组蛋白实验证实其在维持小鼠胚胎干细胞原始多能性中的关键作用，通过抑制分化信号通路(如FGF/ERK)和调控DNA甲基化相关基因(如Dnmt3)的表观遗传状态，确保干细胞的未分化状态。
2. **文献名称**：*PRDM14 interacts with DNA-binding proteins to regulate chromatin modifiers in embryonic stem cells*
**作者**：Chia, N.Y., et al.
**摘要**：通过体外重组PRDM14蛋白与染色质免疫共沉淀(ChIP)技术，研究发现PRDM14直接结合靶基因启动子区域(如NANOG)，并与OCT4/SOX2等转录因子协同作用，招募组蛋白修饰复合物(如Polycomb)，调控多能性相关基因的表达。
3. **文献名称**：*Structural basis of PRDM14-mediated transcriptional regulation in early development*
**作者**：Hörmanser, H., et al.
**摘要**：利用重组PRDM14蛋白进行X射线晶体学分析，解析了其锌指结构域与DNA结合的分子机制，揭示了PRDM14通过特异性识别靶序列调控早期胚胎发育相关基因的分子基础。
**注意**：以上文献标题和作者为示例性质，建议通过PubMed或Google Scholar以“PRDM14 recombinant protein”“PRDM14 structure/function”等关键词检索最新具体文献。
PRDM14 is a transcription factor belonging to the PRDI-BF1-RIZ (PRDM) protein family, characterized by a conserved PR (PRDI-BF1-RIZ) homologous domain with potential methyltransferase activity. It plays critical roles in embryonic development, pluripotency maintenance, and germline specification. PRDM14 regulates epigenetic states by interacting with chromatin modifiers and repressing genes involved in differentiation, thereby preserving the naive pluripotency of embryonic stem cells (ESCs). Its expression is tightly controlled, with high activity in early embryos, primordial germ cells, and certain cancers, particularly germ cell tumors and aggressive malignancies like breast cancer.
Recombinant PRDM14 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells), the recombinant form allows researchers to analyze its DNA-binding specificity, interactions with co-factors (e.g., OCT4. NANOG), and enzymatic functions. Purification methods often involve affinity tags (e.g., His-tag) for efficient isolation. Studies using recombinant PRDM14 have revealed its role in resetting epigenetic marks, such as erasing H3K9me2 and suppressing the DNA damage response during reprogramming.
Its dysregulation is linked to developmental disorders and oncogenesis, making recombinant PRDM14 a tool for drug screening or therapeutic targeting. However, challenges persist in maintaining its structural stability and post-translational modifications in vitro. Ongoing research focuses on optimizing expression systems to better mimic native protein behavior, enhancing its utility in regenerative medicine and cancer biology.
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