| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rock2 |
| Uniprot No | O75116 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 5-554aa |
| 氨基酸序列 | PPTGKMPGAPETAPGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGFYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQKCKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQIST |
| 预测分子量 | 66 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于ROCK2重组蛋白的模拟参考文献(内容基于领域常见研究方向构建)：
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1. **标题**: *"Expression and purification of active recombinant ROCK2 kinase domain in Escherichia coli for biochemical assays"*
**作者**: Yamamoto A, et al.
**摘要**: 本研究报道了通过大肠杆菌表达系统高效制备具有激酶活性的重组ROCK2蛋白(激酶结构域)。优化后的纯化方法可获得高纯度蛋白，并通过体外磷酸化实验验证其催化活性，为药物筛选提供基础工具。
2. **标题**: *"Structural insights into ROCK2 activation mechanism revealed by cryo-EM analysis of recombinant human protein"*
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜技术解析了重组人源ROCK2全长蛋白的构象变化，揭示了其与RhoA结合后的激活机制，为设计特异性抑制剂提供了结构生物学依据。
3. **标题**: *"ROCK2 recombinant protein enhances fibrotic signaling in hepatic stellate cells: Implications for liver fibrosis"*
**作者**: Smith J, et al.
**摘要**: 通过体外实验证明，外源性重组ROCK2蛋白可激活肝星状细胞的促纤维化通路(如COL1A1表达上调)，提示ROCK2在肝纤维化中的直接作用。
4. **标题**: *"Development of a high-throughput screening platform using fluorescent-labeled ROCK2 recombinant protein for kinase inhibitor discovery"*
**作者**: Wang X, et al.
**摘要**: 开发了一种基于荧光标记重组ROCK2蛋白的高通量激酶活性检测体系，成功筛选出多个新型小分子抑制剂，并验证其细胞水平抗迁移效果。
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注：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实发表的论文。
**Background of ROCK2 Recombinant Protein**
ROCK2 (Rho-associated coiled-coil containing protein kinase 2) is a serine/threonine kinase belonging to the ROCK family, which plays a critical role in regulating cytoskeletal dynamics, cell contraction, and motility. It is activated by Rho GTPases, primarily RhoA, and functions as a downstream effector in Rho-mediated signaling pathways. Structurally, ROCK2 contains an N-terminal kinase domain, a central coiled-coil region, and a C-terminal pleckstrin homology (PH) domain, which regulates its autoinhibition and interaction with downstream substrates.
ROCK2 is involved in diverse physiological processes, including vascular smooth muscle contraction, stress fiber formation, and cell adhesion. Dysregulation of ROCK2 has been implicated in pathologies such as hypertension, cardiovascular diseases, fibrosis, and cancer metastasis. Its role in promoting cell migration and invasion makes it a target in oncology research, while its influence on vascular tone links it to cardiovascular therapeutics.
Recombinant ROCK2 proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce highly purified, active kinase for *in vitro* studies. These proteins retain functional domains and catalytic activity, enabling researchers to investigate ROCK2’s biochemical properties, screen inhibitors, or map signaling networks. Recombinant ROCK2 is widely used in drug discovery, particularly for developing inhibitors (e.g., fasudil) to treat diseases driven by aberrant Rho/ROCK signaling. Its application extends to studying molecular mechanisms underlying neurological disorders, inflammation, and tissue remodeling, underscoring its therapeutic and experimental relevance.
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