| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCT2 |
| Uniprot No | P78371-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-535aa |
| 氨基酸序列 | ASLSLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLGPKGMDKI LLSSGRDASLMVTNDGATILKNIGVDNPAAKVLVDMSRVQDDEVGDGTTS VTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSD EVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHI IKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKI FGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPE QLFGAAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEE VMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTV KDSRTVYGGGCSEMLMAHAVTQLANRTPGKEAVAMESYAKALRMLPTIIA DNAGYDSADLVAQLRAAHSEGNTTAGLDMREGTIGDMAILGITESFQVKR QVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇与CCT2重组蛋白相关的文献概括(内容为模拟生成，仅供参考)：
1. **"Recombinant human CCT2 subunit exhibits chaperone activity in vitro"**
*作者：Martín-Benito et al.*
摘要：研究通过大肠杆菌表达系统成功纯化重组人源CCT2蛋白，并验证其在体外辅助β-actin折叠的分子伴侣活性，揭示了CCT2在蛋白质折叠中的独立功能。
2. **"Overexpression of CCT2 promotes hepatocellular carcinoma progression via ERK signaling"**
*作者：Li et al.*
摘要：利用重组CCT2蛋白转染肝癌细胞，发现其过表达通过激活ERK通路促进肿瘤增殖和迁移，为CCT2作为癌症治疗靶点提供依据。
3. **"Structural insights into the CCT2 subunit assembly using recombinant protein technology"**
*作者：Yam et al.*
摘要：通过重组表达CCT2及其突变体，结合冷冻电镜技术解析CCT2在完整CCT复合体中的构象变化，阐明其与其他亚基的互作机制。
4. **"CCT2 interacts with misfolded α-synuclein in Parkinson's disease models"**
*作者：Wang et al.*
摘要：利用重组CCT2蛋白进行免疫共沉淀实验，发现其与α-突触核蛋白异常聚集体特异性结合，提示其在神经退行性疾病中的病理作用。
注：以上文献信息为示例性质，实际研究请通过学术数据库检索确认。
CCT2 (Chaperonin Containing TCP-1 subunit 2) is a critical component of the eukaryotic chaperonin complex known as CCT (or TRiC), which plays a central role in facilitating the folding of newly synthesized polypeptides, particularly cytoskeletal proteins like actin and tubulin. As one of eight paralogous subunits (CCT1-8) that form the double-ring structure of CCT, CCT2 contributes to the ATP-dependent conformational changes required for substrate binding, encapsulation, and release. The CCT complex operates through a coordinated mechanism where each subunit interacts with specific regions of client proteins, ensuring proper folding and cellular function.
Recombinant CCT2 protein is engineered using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to study its structural and functional properties in isolation or within reconstituted CCT complexes. Its production often involves affinity tags (e.g., His-tag) for purification, followed by biochemical validation to ensure native-like activity. Research on recombinant CCT2 has shed light on its unique substrate recognition motifs, ATPase activity, and cooperative interactions with other CCT subunits. Dysregulation of CCT2 expression has been implicated in cancer progression, neurodegenerative disorders, and developmental defects, making it a potential therapeutic target. Studies utilizing recombinant CCT2 have advanced structural biology techniques (e.g., cryo-EM) and drug discovery efforts aimed at modulating chaperonin function. Its evolutionary conservation across species underscores its fundamental role in maintaining proteostasis, while engineered variants continue to reveal mechanistic insights into chaperonin-mediated folding pathways.
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