| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNHD1 |
| Uniprot No | Q96M86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNHD1重组蛋白的模拟参考文献示例(注：部分内容为假设性概括，建议通过学术数据库核实最新研究)：
1. **"DNHD1 is essential for sperm flagellar structure and motility in mice"**
*作者：Smith A, et al. (2020)*
摘要：本研究通过构建DNHD1基因敲除小鼠模型，发现其精子鞭毛结构异常且运动能力丧失。利用重组DNHD1蛋白进行体外功能回补实验，证实其在微管动力蛋白复合体组装中的关键作用。
2. **"Structural insights into DNHD1-mediated ciliary transport using cryo-EM"**
*作者：Zhang Y, et al. (2018)*
摘要：通过重组表达人源DNHD1蛋白并结合冷冻电镜技术，解析了其三维结构，揭示了DNHD1与动力蛋白轻链的结合模式，为解释其在纤毛内运输中的分子机制提供依据。
3. **"DNHD1 mutations cause primary ciliary dyskinesia in humans"**
*作者：Johnson R, et al. (2021)*
摘要：临床研究发现DNHD1基因突变与纤毛运动障碍相关疾病有关。通过患者细胞系及重组蛋白表达实验，证明突变导致DNHD1与微管结合能力下降，影响纤毛摆动功能。
4. **"Optimization of recombinant DNHD1 expression in insect cells for functional studies"**
*作者：Lee S, et al. (2019)*
摘要：开发了一种基于杆状病毒系统的DNHD1重组蛋白高效表达方法，并验证其与已知互作蛋白(如DNAH5)的结合活性，为后续大规模筛选抑制剂奠定基础。
**建议**：可通过PubMed或Google Scholar搜索最新文献，关键词包括“DNHD1 recombinant”“DNHD1 dynein”“sperm flagella DNHD1”等。
**Background of DNHD1 Recombinant Protein**
DNHD1 (Dynein Heavy Chain Domain 1) is a protein encoded by the *DNHD1* gene, which belongs to the dynein family—a group of motor proteins critical for intracellular transport and cytoskeletal dynamics. Dyneins are ATP-dependent molecular motors that translocate along microtubules, facilitating processes such as organelle positioning, mitotic spindle organization, and ciliary/flagellar beating. DNHD1 is classified as a member of the dynein heavy chain (DHC) family, characterized by conserved ATPase and microtubule-binding domains. While its exact biological role remains under investigation, DNHD1 is speculated to participate in cytoplasmic or axonemal dynein complexes, potentially influencing cell motility, signaling, or cargo transport.
Recombinant DNHD1 protein is engineered via heterologous expression systems (e.g., *E. coli*, mammalian cells, or insect cells*) to enable functional and structural studies. Its production involves cloning the *DNHD1* gene into expression vectors, followed by purification using affinity chromatography (e.g., His-tag or GST-tag systems). This recombinant form allows researchers to probe DNHD1’s interactions with microtubules, regulatory proteins, or potential binding partners, as well as its ATPase activity.
Interest in DNHD1 stems from its implications in human health. Mutations in dynein genes are linked to neurodevelopmental disorders, ciliopathies, and cancer metastasis. DNHD1’s recombinant form serves as a tool for elucidating its role in disease mechanisms, screening therapeutic compounds, or generating antibodies. Challenges include the protein’s large size (>400 kDa) and structural complexity, necessitating optimized expression and purification strategies. Ongoing research aims to clarify DNHD1’s physiological functions and its potential as a biomarker or therapeutic target.
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