Recombinant Human CENPP protein

中文名： 着丝粒蛋白P(CENPP)重组蛋白
别名： CENPP；Centromere protein P

货号: PA1000-579DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	CENPP
Uniprot No	Q6IPU0
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-288aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MDAELAEVRA LQAEIAALRR ACEDPPAPWE EKSRVQKSFQ AIHQFNLEGW KSSKDLKNQL GHLESELSFL STLTGINIRN HSKQTEDLTS TEMTEKSIRK VLQRHRLSGN CHMVTFQLEF QILEIQNKER LSSAVTDLNI IMEPTECSEL SEFVSRAEER KDLFMFFRSL HFFVEWFEYR KRTFKHLKEK YPDAVYLSEG PSSCSMGIRS ASRPGFELVI VWRIQIDEDG KVFPKLDLLT KVPQRALELD KNRAIETAPL SFRTLVGLLG IEAALESLIK SLCAEENN
预测分子量	35 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于CENPP重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：

1. **文献名称**：Structural Insights into CENPP Recombinant Protein and Its Role in Centromere Assembly

**作者**：Smith J. et al.

**摘要**：本研究通过重组表达技术获得人源CENPP蛋白，并利用X射线晶体学解析其三维结构。结果表明，CENPP通过特定的α-螺旋结构域与CENP-Q/L复合物相互作用，为阐明其在着丝粒组装中的分子机制提供了依据。

2. **文献名称**：Functional Characterization of Recombinant CENPP in Chromosome Segregation

**作者**：Tanaka R. et al.

**摘要**：通过体外重建实验发现，重组CENPP蛋白可增强有丝分裂中动粒与微管的结合稳定性，敲低CENPP导致染色体分离异常。研究证实CENPP是维持基因组完整性的关键因子。

3. **文献名称**：CENPP Recombinant Protein Interaction Network in Cancer Cell Lines

**作者**：Lee S. et al.

**摘要**：利用重组CENPP蛋白进行免疫共沉淀-质谱分析，鉴定出与CENPP结合的多个新互作蛋白(如MIS18BP1)，并发现其在乳腺癌细胞中过表达可能促进染色体不稳定性及肿瘤进展。

4. **文献名称**：Development of a CENPP Recombinant Protein-Based Diagnostic Assay for Aneuploidy Screening

**作者**：Garcia M. et al.

**摘要**：基于重组CENPP蛋白的高效表达和纯化，开发了一种新型血清学检测方法，用于早期筛查胚胎发育异常中的非整倍体现象，临床样本验证显示其灵敏度和特异性均优于传统标记物。

注：以上内容为模拟生成，实际文献需通过PubMed或Web of Science等数据库检索确认。

背景信息

**Background of CENPP Recombinant Protein**

CENPP (Centromere Protein P) is a component of the constitutive centromere-associated network (CCAN), a multi-protein complex essential for kinetochore assembly and chromosome segregation during mitosis and meiosis. As part of the CCAN, CENPP localizes to the inner centromere region and interacts with other centromere proteins, such as CENP-A, CENP-C, and CENP-N, to stabilize the kinetochore-microtubule interface and ensure accurate chromosome partitioning. Dysregulation of centromere proteins, including CENPP, is linked to genomic instability, aneuploidy, and cancer progression.

Recombinant CENPP protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional CENPP for biochemical and cellular studies. Its recombinant form enables researchers to explore CENPP's structural interactions within the CCAN, its role in centromere identity maintenance, and its contribution to mitotic fidelity. Studies utilizing recombinant CENPP have shed light on how mutations or aberrant expression of centromere proteins disrupt cell division, potentially driving carcinogenesis. Additionally, it serves as a tool for screening therapeutic agents targeting centromere dysfunction in cancers.

The development of CENPP recombinant protein has advanced mechanistic insights into centromere biology, offering a platform to dissect molecular pathways underlying chromosomal disorders and cancer. Its application extends to diagnostics and targeted therapies aimed at correcting mitotic errors in proliferative diseases.