| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IFIH1 |
| Uniprot No | Q9BYX4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 700-1025aa |
| 氨基酸序列 | KLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED |
| 预测分子量 | 41.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IFIH1重组蛋白的3篇参考文献，涵盖结构解析、功能机制及疾病相关性研究：
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1. **文献名称**：*Structural basis of viral RNA recognition and activation of innate immune receptor MDA5*
**作者**：Wu B et al.
**摘要**：本研究通过表达重组IFIH1(MDA5)蛋白，解析了其与病毒RNA结合的晶体结构，揭示了MDA5通过C端结构域识别长双链RNA的分子机制，并阐明了其激活下游抗病毒信号通路(如MAVS通路)的关键构象变化。
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2. **文献名称**：*Functional roles of IFIH1 polymorphisms in autoimmune diabetes*
**作者**：Smyth DJ et al.
**摘要**：文章利用重组IFIH1突变体蛋白进行功能实验，发现某些单核苷酸多态性(如rs1990760)会增强MDA5对RNA的亲和力，导致过度免疫反应，从而增加1型糖尿病和自身免疫疾病风险，为基因变异与疾病关联提供了分子证据。
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3. **文献名称**：*Recombinant IFIH1 protein production and its application in antiviral drug screening*
**作者**：Jiang F et al.
**摘要**：研究优化了IFIH1重组蛋白在大肠杆菌中的表达与纯化流程，并基于该蛋白建立高通量筛选平台，发现小分子化合物可抑制MDA5-RNA相互作用，为开发抗病毒或自身免疫疾病药物提供了潜在靶点。
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**注**：以上文献信息为示例性质，实际引用时需核实具体来源及细节。如需精准文献，建议通过PubMed或Google Scholar检索关键词“IFIH1 recombinant protein”或“MDA5 structural study”获取最新研究。
IFIH1 (Interferon Induced With Helicase C Domain 1), also known as MDA5 (melanoma differentiation-associated protein 5), is a cytoplasmic RNA helicase encoded by the IFIH1 gene. It belongs to the RIG-I-like receptor (RLR) family, playing a critical role in innate immunity by detecting viral double-stranded RNA (dsRNA) during infections. Upon activation, IFIH1 triggers downstream signaling cascades via the mitochondrial antiviral-signaling protein (MAVS), leading to the production of type I interferons (IFNs) and proinflammatory cytokines, which orchestrate antiviral defenses.
The recombinant IFIH1 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or mammalian cell systems to ensure proper folding and functionality. Purified recombinant IFIH1 retains its ATPase and RNA-binding activities, making it valuable for studying viral recognition mechanisms, immune signaling pathways, and autoimmune disorders. Notably, gain-of-function mutations in IFIH1 are linked to autoimmune diseases like Aicardi-Goutières syndrome and type 1 diabetes, while loss-of-function variants may increase susceptibility to viral infections.
Research applications include in vitro RNA-binding assays, structural studies (e.g., cryo-EM analyses of MDA5-dsRNA complexes), and drug screening for antiviral or immunomodulatory therapies. Its recombinant form also serves as a tool to investigate the balance between immune activation and tolerance, providing insights into therapeutic strategies for autoimmune conditions and antiviral interventions.
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