| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHA1 |
| Uniprot No | Q9HB21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404aa |
| 氨基酸序列 | MPYVDRQNRI CGFLDIEENE NSGKFLRRYF ILDTREDSFV WYMDNPQNLP SGSSRVGAIK LTYISKVSDA TKLRPKAEFC FVMNAGMRKY FLQANDQQDL VEWVNVLNKA IKITVPKQSD SQPNSDNLSR HGECGKKQVS YRTDIVGGVP IITPTQKEEV NECGESIDRN NLKRSQSHLP YFTPKPPQDS AVIKAGYCVK QGAVMKNWKR RYFQLDENTI GYFKSELEKE PLRVIPLKEV HKVQECKQSD IMMRDNLFEI VTTSRTFYVQ ADSPEEMHSW IKAVSGAIVA QRGPGRSASS EHPPGPSESK HAFRPTNAAT ATSHSTASRS NSLVSTFTME KRGFYESLAK VKPGNFKVQT VSPREPASKV TEQALLRPQS KNGPQEKDCD LVDLDDASLP VSDV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLEKHA1重组蛋白的3篇参考文献的简要列举(注：内容基于模拟生成，非真实文献)：
1. **文献名称**：*"Recombinant PLEKHA1 regulates cell-cell adhesion through interaction with p120-catenin"*
**作者**：Smith A, et al.
**摘要**：研究通过在大肠杆菌中表达重组PLEKHA1蛋白，发现其通过结合p120-catenin调控上皮细胞间黏附连接，揭示了其在维持细胞极性中的作用。
2. **文献名称**：*"Expression and functional characterization of PLEKHA1 in TGF-β signaling pathway"*
**作者**：Zhang L, et al.
**摘要**：利用HEK293细胞表达重组PLEKHA1蛋白，证实其通过调控TGF-β受体稳定性影响下游信号通路，可能参与纤维化疾病的发生。
3. **文献名称**：*"PLEKHA1 knockout via CRISPR/Cas9 alters angiogenesis in endothelial cells"*
**作者**：Tanaka K, et al.
**摘要**：通过重组PLEKHA1蛋白回补实验，证明PLEKHA1缺失导致血管内皮细胞迁移和血管生成能力下降，提示其在血管发育中的关键功能。
(以上内容为示例，实际文献需通过PubMed或Google Scholar等平台检索确认。)
PLEKHA1 (Pleckstrin Homology Domain Containing A1) is a multidomain protein involved in regulating cell-cell adhesion, polarity, and intracellular signaling. Initially identified through homology to pleckstrin homology (PH) domain-containing proteins, it localizes to the apical region of epithelial cells and interacts with components of the Par complex (e.g., PAR3. PAR6. and aPKC), which governs cell polarity. Structurally, PLEKHA1 contains an N-terminal PH domain that binds phosphoinositides, enabling membrane association, and a C-terminal coiled-coil domain mediating protein-protein interactions. Its role in maintaining epithelial integrity links it to pathological processes, including cancer progression, where disrupted polarity often drives metastasis.
Recombinant PLEKHA1 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Purified recombinant PLEKHA1 retains structural domains critical for studying its interactions with signaling molecules (e.g., Cdc42. microtubules) or testing its influence on cell adhesion pathways. Researchers employ it to dissect mechanisms underlying diseases like hypertension (via vascular remodeling) or age-related macular degeneration (linked to PLEKHA1 polymorphisms). Tagged versions (e.g., His, GST, FLAG) facilitate pull-down assays, while truncated mutants help map domain-specific functions. Recent studies also explore PLEKHA1’s potential as a biomarker or therapeutic target, particularly in cancers where its expression correlates with clinical outcomes. However, its pleiotropic roles and tissue-specific signaling effects necessitate careful experimental design when using recombinant forms. Quality control metrics (purity >90%, endotoxin levels, activity validation) ensure reliability in assays ranging from in vitro binding to cell-based functional rescue experiments.
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