| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITFG1 |
| Uniprot No | Q8TB96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-612aa |
| 氨基酸序列 | MAAAGRLPSSWALFSPLLAGLALLGVGPVPARALHNVTAELFGAEAWGTLAAFGDLNSDKQTDLFVLRERNDLIVFLADQNAPYFKPKVKVSFKNHSALITSVVPGDYDGDSQMDVLLTYLPKNYAKSELGAVIFWGQNQTLDPNNMTILNRTFQDEPLIMDFNGDLIPDIFGITNESNQPQILLGGNLSWHPALTTTSKMRIPHSHAFIDLTEDFTADLFLTTLNATTSTFQFEIWENLDGNFSVSTILEKPQNMMVVGQSAFADFDGDGHMDHLLPGCEDKNCQKSTIYLVRSGMKQWVPVLQDFSNKGTLWGFVPFVDEQQPTEIPIPITLHIGDYNMDGYPDALVILKNTSGSNQQAFLLENVPCNNASCEEARRMFKVYWELTDLNQIKDAMVATFFDIYEDGILDIVVLSKGYTKNDFAIHTLKNNFEADAYFVKVIVLSGLCSNDCPRKITPFGVNQPGPYIMYTTVDANGYLKNGSAGQLSQSAHLALQLPYNVLGLGRSANFLDHLYVGIPRPSGEKSIRKQEWTAIIPNSQLIVIPYPHNVPRSWSAKLYLTPSNIVLLTAIALIGVCVFILAIIGILHWQEKKADDREKRQEAHRFHFDAM |
| 预测分子量 | 68,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITFG1重组蛋白的模拟参考文献示例(注：部分文献信息为假设，建议通过学术数据库核实具体研究)：
1. **文献名称**: "Efficient Expression and Purification of Recombinant Human ITFG1 in a Mammalian System"
**作者**: Chen, L., et al.
**摘要**: 本研究报道了在HEK293细胞中高效表达并纯化人源ITFG1重组蛋白的方法。通过优化载体构建和培养条件，获得了高纯度蛋白，并验证了其与整合素β1亚基的结合活性，为后续功能研究奠定基础。
2. **文献名称**: "ITFG1 Recombinant Protein Suppresses Tumor Cell Migration via Modulating Focal Adhesion Kinase Signaling"
**作者**: Kim, S., et al.
**摘要**: 利用昆虫表达系统制备ITFG1重组蛋白，发现其通过抑制黏着斑激酶(FAK)磷酸化，显著降低多种癌细胞迁移能力，提示ITFG1可能作为肿瘤转移的潜在干预靶点。
3. **文献名称**: "Structural Characterization of ITFG1 Reveals a Novel Role in Extracellular Matrix Remodeling"
**作者**: Müller, R., et al.
**摘要**: 通过大肠杆菌表达系统获得ITFG1胞外域重组蛋白，结合X射线晶体学解析其三维结构，发现其FG-GAP结构域参与胶原结合，可能调控细胞外基质重塑过程。
4. **文献名称**: "Development of a Polyclonal Antibody against Recombinant ITFG1 for Immunohistochemical Applications"
**作者**: Gupta, P., et al.
**摘要**: 研究利用原核表达纯化的ITFG1重组蛋白制备多克隆抗体，验证其在组织切片中的特异性染色，发现ITFG1在肾脏和肺组织中的高表达模式，暗示其器官发育中的功能。
**备注**：以上文献为基于ITFG1相关研究的合理推测，实际文献可能需要通过PubMed、Web of Science等平台以关键词“ITFG1 recombinant”“ITFG1 expression”检索。真实研究可能集中于ITFG1在细胞黏附、癌症或器官发育中的功能机制。
ITFG1 (Integrin Alpha FG-GAP Repeat-Containing 1) is a protein encoded by the ITFG1 gene, which belongs to the integrin alpha chain family. It is characterized by the presence of FG-GAP repeats, structural motifs involved in protein-protein interactions and cellular adhesion processes. ITFG1 is ubiquitously expressed in human tissues, with notable roles in cell adhesion, migration, and signal transduction. Studies suggest its involvement in integrin-mediated pathways, potentially regulating extracellular matrix (ECM) interactions and cytoskeletal organization. Dysregulation of ITFG1 has been linked to various pathologies, including cancer progression, immune disorders, and neurological conditions, highlighting its biological significance.
Recombinant ITFG1 protein is engineered using heterologous expression systems, such as Escherichia coli or mammalian cell lines (e.g., HEK293), to ensure proper folding and post-translational modifications. The recombinant form typically retains functional domains critical for binding partners, enabling in vitro studies of its molecular interactions. Researchers utilize ITFG1 recombinant proteins to investigate mechanisms of integrin signaling, screen therapeutic compounds targeting adhesion-related diseases, and develop diagnostic antibodies. Its applications extend to structural biology (e.g., crystallography) and functional assays (e.g., cell adhesion inhibition studies). Quality control involves SDS-PAGE, Western blotting, and mass spectrometry to verify purity and authenticity. As a research tool, recombinant ITFG1 facilitates mechanistic insights into cell-ECM communication and disease pathways while offering potential for therapeutic intervention strategies.
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