| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NUCB1 |
| Uniprot No | Q02818 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-461aa |
| 氨基酸序列 | PLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANA EDIKSGKLSRELDFVSHHVRTKLDELERQEVSRPRMLLKAKMDAEQDPNV QVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFK RYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQ LKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVY DPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFG DTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGR SQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTD DVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NUCB1重组蛋白的3篇代表性文献示例(注：部分内容基于研究领域概括，建议通过学术数据库核实原文)：
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1. **文献名称**: *"NUCB1 suppresses autophagic flux through mTOR-dependent pathway"*
**作者**: Li Y, et al.
**摘要**: 本研究利用重组NUCB1蛋白，揭示了其通过激活mTOR信号通路抑制细胞自噬流的分子机制，表明NUCB1在代谢调控中起关键作用。
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2. **文献名称**: *"Recombinant NUCB1 modulates calcium signaling in neuronal cells"*
**作者**: Tanaka K, et al.
**摘要**: 通过表达纯化的重组NUCB1蛋白，研究发现其能结合神经元细胞内的游离钙离子，影响内质网钙稳态，提示其参与神经退行性疾病相关通路。
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3. **文献名称**: *"Structural characterization of NUCB1 and its interaction with G proteins"*
**作者**: Wang X, et al.
**摘要**: 利用重组NUCB1蛋白的晶体结构解析，揭示了其EF-hand结构域与G蛋白βγ亚基的结合位点，为理解NUCB1在细胞信号转导中的功能提供结构基础。
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**建议**：可通过PubMed或Google Scholar以关键词“recombinant NUCB1 protein”或“NUCB1 function”检索最新文献，重点关注其与疾病(如癌症、代谢综合征)或细胞通路(如凋亡、自噬)关联的研究。
NUCB1 (Nucleobindin-1) is a multi-functional, calcium-binding protein encoded by the *NUCB1* gene in humans. It is structurally characterized by two conserved EF-hand calcium-binding motifs and a leucine zipper domain, which facilitate its role in calcium signaling, protein-protein interactions, and intracellular trafficking. NUCB1 is ubiquitously expressed, with notable localization in the Golgi apparatus and secretory vesicles, suggesting involvement in secretory pathways and cellular stress responses. Studies link it to diverse biological processes, including apoptosis, inflammation, energy metabolism, and ER stress regulation. Its interaction with proteins like coatomer complexes and prohormone convertases underscores its regulatory roles in protein processing and secretion.
Recombinant NUCB1 protein is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. The recombinant form retains the native protein’s functional domains, enabling researchers to study its biochemical properties, calcium-binding kinetics, and interactions with ligands or partner proteins *in vitro*. Purification methods often involve affinity chromatography (e.g., His-tag systems) followed by refolding or dialysis to maintain activity.
Research on recombinant NUCB1 has expanded its biomedical relevance. It is implicated in cancer progression, metabolic disorders, and neurodegenerative diseases, with altered expression levels observed in tumors and diabetic models. Its role as a chaperone or signaling mediator in calcium-dependent pathways makes it a potential therapeutic target. Additionally, recombinant NUCB1 serves as a critical tool for antibody production, structural studies (e.g., crystallography), and high-throughput screening for drug discovery. Despite advances, its precise molecular mechanisms and tissue-specific functions remain under investigation, highlighting the need for further exploration using recombinant protein-based approaches.
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