| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCOA3 |
| Uniprot No | Q9Y6Q9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1424aa |
| 氨基酸序列 | MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC |
| 预测分子量 | 155,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NCOA3重组蛋白的虚构参考文献示例，格式符合要求：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant NCOA3 in Hormone Signaling*
**作者**：Zhang, L. et al.
**摘要**：本研究利用大肠杆菌系统表达并纯化了重组NCOA3蛋白，通过X射线晶体学解析其C端转录激活域结构，揭示了其与核受体(如雌激素受体)的相互作用机制，为靶向NCOA3的癌症治疗提供结构基础。
2. **文献名称**：*Recombinant NCOA3 Enhances Transcriptional Coactivation in Breast Cancer Cell Models*
**作者**：Martinez, R. & Lee, S.K.
**摘要**：通过哺乳动物细胞系表达重组NCOA3.研究证实其过表达显著增强雌激素依赖的基因转录活性，并促进乳腺癌细胞增殖，提示其在激素依赖性肿瘤中的致癌作用。
3. **文献名称**：*Purification and Biochemical Analysis of NCOA3 Recombinant Protein for Drug Screening*
**作者**：Tanaka, H. et al.
**摘要**：开发了一种高效昆虫细胞表达系统制备重组NCOA3蛋白，结合高通量筛选技术鉴定出小分子抑制剂，可阻断NCOA3与p300/CBP的相互作用，为开发抗癌药物奠定基础。
4. **文献名称**：*NCOA3 Recombinant Mutants Reveal Key Domains for Coactivator Recruitment*
**作者**：Wang, Y. & Greene, G.L.
**摘要**：通过构建NCOA3重组蛋白的突变体(如ΔLXXLL)，验证其与核受体(AR、ERα)的结合能力，证明LXXLL基序是招募受体的关键区域，为功能域研究提供实验依据。
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注：以上内容为模拟示例，实际文献需通过学术数据库检索确认。
**Background of NCOA3 Recombinant Protein**
NCOA3 (Nuclear Receptor Coactivator 3), also known as AIB1. SRC-3. or ACTR, is a member of the steroid receptor coactivator (SRC) family. It functions as a transcriptional coactivator for nuclear receptors and other transcription factors, playing a critical role in regulating gene expression involved in cell proliferation, differentiation, and metabolism. Structurally, NCOA3 contains multiple functional domains, including basic helix-loop-helix (bHLH) motifs, receptor interaction domains, and activation domains, enabling interactions with chromatin-modifying enzymes and the transcriptional machinery.
In physiological contexts, NCOA3 is essential for hormone-dependent signaling pathways, particularly those mediated by estrogen, androgen, and thyroid hormone receptors. Dysregulation of NCOA3 is strongly linked to cancer. Amplification or overexpression of NCOA3 is observed in breast, prostate, and ovarian cancers, where it promotes tumor growth, metastasis, and therapy resistance by enhancing oncogenic transcription programs. Its role in metabolic diseases and developmental disorders is also under investigation.
Recombinant NCOA3 protein is engineered to study its molecular interactions, post-translational modifications, and functional mechanisms *in vitro*. Produced via bacterial or mammalian expression systems, it retains functional domains and is often tagged (e.g., His, GST) for purification and detection. Researchers utilize this protein to investigate coactivator recruitment, chromatin remodeling, and drug-target interactions, aiding the development of therapies targeting NCOA3-overexpressing cancers.
Overall, NCOA3 recombinant protein serves as a vital tool for dissecting transcriptional regulation and advancing precision oncology strategies.
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