| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NURR1 |
| Uniprot No | P43354 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598aa |
| 氨基酸序列 | MPCVQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTE ITATTSLPSFSTFMDNYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNY QQHSHLPPQSEEMMPHSGSVYYKPSSPPTPTTPGFQVQHSPMWDDPGSLH NFHQNYVATTHMIEQRKTPVSRLSLFSFKQSPPGTPVSSCQMRFDGPLHV PMNPEPAGSHHVVDGQTFAVPNPIRKPASMGFPGLQIGHASQLLDTQVPS PPSRGSPSNEGLCAVCGDNAACQHYGVRTCEGCKGFFKRTVQKNAKYVCL ANKNCPVDKRRRNRCQYCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPK SPQEPSPPSPPVSLISALVRAHVDSNPAMTSLDYSRFQANPDYQMSGDDT QHIQQFYDLLTGSMEIIRGWAEKIPGFADLPKADQDLLFESAFLELFVLR LAYRSNPVEGKLIFCNGVVLHRLQCVRGFGEWIDSIVEFSSNLQNMNIDI SAFSCIAALAMVTERHGLKEPKRVEELQNKIVNCLKDHVTFNNGGLNRPN YLSKLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPAIIDKLFLDTLPF |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NURR1重组蛋白的3篇代表性文献概览(基于公开信息模拟，非真实文献)：
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1. **文献名称**: *Structural basis of Nurr1 DNA binding and activation in dopaminergic neurons*
**作者**: Wang Z, et al.
**摘要**: 本研究解析了重组NURR1蛋白的DNA结合结构域晶体结构，揭示了其与靶基因启动子中特定响应元件的结合模式，阐明了NURR1通过构象变化调控多巴胺能神经元分化的分子机制。
2. **文献名称**: *Nurr1-reprogrammed progenitor cells for Parkinson's disease therapy*
**作者**: Kim CH, et al.
**摘要**: 利用重组NURR1蛋白与特定小分子协同处理，将人多能干细胞定向分化为功能性多巴胺能神经元，证明了其在帕金森病细胞替代治疗中的潜在应用价值。
3. **文献名称**: *Recombinant Nurr1-LXRβ fusion protein attenuates neuroinflammation via transcriptional regulation*
**作者**: Saijo K, et al.
**摘要**: 构建NURR1与LXRβ的融合重组蛋白，发现其通过双重调控神经炎症相关基因(如IL-6、TNF-α)的转录活性，显著改善帕金森病小鼠模型的神经退行性病变。
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注：以上内容为模拟示例，实际文献需通过PubMed/Google Scholar等平台检索(关键词：NURR1 recombinant protein, dopamine neurons, Parkinson's disease)。真实经典文献可能涉及：
- Zetterström RH, et al. *Science* (1997) 首次克隆NURR1基因
- Decressac M, et al. *Cell Stem Cell* (2013) 干细胞分化研究
Nuclear receptor-related 1 protein (NURR1), also known as NR4A2. is a transcription factor belonging to the NR4A subfamily of nuclear receptors. It plays a critical role in the development, maintenance, and function of dopaminergic neurons in the central nervous system, particularly in the substantia nigra—a region implicated in Parkinson’s disease. NURR1 regulates the expression of genes essential for dopamine synthesis, such as tyrosine hydroxylase and dopamine transporter, and is involved in neuroprotection against oxidative stress and inflammation. Dysregulation of NURR1 has been linked to neurodegenerative disorders, psychiatric conditions, and some cancers.
Recombinant NURR1 protein is engineered for in vitro and in vivo studies to elucidate its molecular mechanisms and therapeutic potential. Produced via heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*), the recombinant protein retains functional domains, including the DNA-binding domain (DBD) and ligand-binding domain (LBD), though its endogenous ligand remains unidentified. Researchers utilize this protein to investigate interactions with co-regulators, DNA response elements, or small molecules that modulate its activity. For instance, agonists enhancing NURR1 transcriptional activity are explored as potential neuroprotective agents.
Challenges in working with recombinant NURR1 include its susceptibility to degradation, dependence on post-translational modifications for full functionality, and the need for proper folding to mimic native conformation. Despite these hurdles, it serves as a vital tool for drug screening, structural studies (e.g., X-ray crystallography), and gene therapy approaches aiming to restore dopaminergic signaling in Parkinson’s disease. Recent advances in protein engineering and CRISPR-based delivery systems have amplified its utility in both basic research and translational applications.
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