| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNASE9 |
| Uniprot No | P60153 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-205aa |
| 氨基酸序列 | QEVD TDFDFPEEDK KEEFEECLEK FFSTGPARPP TKEKVKRRVL IEPGMPLNHI EYCNHEIMGK NVYYKHRWVA EHYFLLMQYD ELQKICYNRF VPCKNGIRKC NRSKGLVEGV YCNLTEAFEI PACKYESLYR KGYVLITCSW QNEMQKRIPH TINDLVEPPE HRSFLSEDGV FVISP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNASE9重组蛋白的3篇代表性文献及其摘要概括：
1. **《RNASE9在人类生殖道中的抗菌活性研究》**(作者：Zhang J, et al.)
摘要：研究首次证实RNASE9在男性生殖道上皮细胞中高表达，重组蛋白在体外对大肠杆菌等泌尿致病菌具有直接杀菌作用，提示其在黏膜先天免疫中的潜在作用。
2. **《重组RNASE9蛋白的表达及功能表征》**(作者：Spencer JD, et al.)
摘要：通过原核系统成功表达重组RNASE9.发现其虽缺乏传统RNase活性，但能通过破坏细菌膜结构抑制病原体生长，为开发新型抗菌剂提供理论基础。
3. **《RNASE9进化保守性与抗菌功能的关系》**(作者：Harder J, Schröder JM)
摘要：通过比较基因组学分析，揭示RNASE9在灵长类中具有高度保守性，重组蛋白实验显示其抗菌活性与特定结构域相关，暗示其在宿主防御中的古老起源。
注：上述文献信息为示例性概括，实际引用需通过PubMed或Google Scholar等平台核实具体标题、作者及出版信息。部分早期研究可能集中于基因定位或基础表达分析，建议结合关键词"RNASE9 recombinant protein"+"antimicrobial"进行精准检索。
RNASE9 (Ribonuclease 9) is a member of the RNase A superfamily, a group of proteins characterized by their enzymatic ability to cleave RNA. Initially identified in the human epididymis, RNASE9 is primarily expressed in the male reproductive tract, particularly in the epididymal epithelium. Unlike some canonical RNases (e.g., RNASE1 or RNASE5), RNASE9 exhibits unique structural features and limited catalytic activity due to amino acid substitutions in its catalytic site. This suggests potential non-enzymatic biological roles, possibly related to host defense or sperm maturation.
Recombinant RNASE9 protein is produced via genetic engineering, often using bacterial (e.g., *E. coli*) or mammalian expression systems. The protein retains key structural motifs, including conserved cysteine residues that form disulfide bonds critical for stability. Studies indicate RNASE9 may function as an antimicrobial agent, disrupting bacterial membranes through its cationic properties. Its expression in the epididymis—a site vulnerable to infection—supports a role in innate immunity, protecting spermatozoa and reproductive tissues from pathogens.
Research on recombinant RNASE9 focuses on elucidating its mechanism of action, exploring therapeutic applications in urogenital infections, and investigating its involvement in male infertility. Despite progress, its precise physiological role remains debated, partly due to interspecies variability and context-dependent activity. Ongoing work aims to clarify its interactions with microbial membranes and potential synergies with other antimicrobial peptides. Recombinant RNASE9 also serves as a tool for structural studies, aiding in the design of novel antimicrobial agents inspired by its unique properties.
×
