| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NXN |
| Uniprot No | Q6DKJ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-435aa |
| 氨基酸序列 | SGFLEELLG EKLVTGGGEE VDVHSLGARG ISLLGLYFGC SLSAPCAQLS ASLAAFYGRL RGDAAAGPGP GAGAGAAAEP EPRRRLEIVF VSSDQDQRQW QDFVRDMPWL ALPYKEKHRK LKLWNKYRIS NIPSLIFLDA TTGKVVCRNG LLVIRDDPEG LEFPWGPKPF REVIAGPLLR NNGQSLESSS LEGSHVGVYF SAHWCPPCRS LTRVLVESYR KIKEAGQNFE IIFVSADRSE ESFKQYFSEM PWLAVPYTDE ARRSRLNRLY GIQGIPTLIM LDPQGEVITR QGRVEVLNDE DCREFPWHPK PVLELSDSNA AQLNEGPCLV LFVDSEDDGE SEAAKQLIQP IAEKIIAKYK AKEEEAPLLF FVAGEDDMTD SLRDYTNLPE AAPLLTILDM SARAKYVMDV EEITPAIVEA FVNDFLAEKL KPEPI |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NXN(Nucleoredoxin)重组蛋白研究的3篇模拟参考文献(非真实文献，仅供示例)：
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1. **文献名称**：*Nucleoredoxin regulates Wnt signaling via disulfide bond formation of Dishevelled*
**作者**：Smith A, et al.
**摘要**：本研究揭示了NXN重组蛋白通过催化Dishevelled(Dvl)蛋白的二硫键形成，调控Wnt/β-catenin信号通路的分子机制。实验表明，NXN的氧化还原酶活性对胚胎发育和癌细胞迁移具有关键作用。
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2. **文献名称**：*Structural and functional analysis of recombinant Nucleoredoxin in oxidative stress response*
**作者**：Tanaka K, et al.
**摘要**：通过X射线晶体学解析了重组NXN蛋白的三维结构，发现其CXXC基序是氧化还原活性的核心。功能实验证明NXN通过抑制ROS(活性氧)积累保护细胞免受氧化损伤，并参与p53信号通路的调控。
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3. **文献名称**：*Nucleoredoxin knockout mice exhibit neurodevelopmental defects and altered redox homeostasis*
**作者**：Chen L, et al.
**摘要**：构建了NXN基因敲除小鼠模型，发现其出现海马区神经元发育异常和运动功能障碍。蛋白质组学分析表明，NXN缺失导致硫氧还蛋白家族(Thioredoxin)代谢失衡，提示其在神经退行性疾病中的潜在作用。
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(注：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science检索真实文献。)
NXN (Nucleoredoxin) is a redox-sensitive protein belonging to the thioredoxin superfamily, primarily involved in regulating cellular redox homeostasis and signaling pathways. Initially identified for its nuclear localization, it is now known to function in both the nucleus and cytoplasm. Structurally, NXN contains a conserved thioredoxin-like domain with a CXXC active site motif, enabling its participation in redox reactions through disulfide bond exchange. Unlike classical thioredoxins, NXN exhibits unique interactions with signaling molecules, particularly within the Wnt/β-catenin pathway, where it modulates Dishevelled (Dvl) protein activity, influencing cell proliferation and differentiation.
Recombinant NXN protein is produced using expression systems like *E. coli* or mammalian cells, followed by purification techniques such as affinity chromatography. Its recombinant form retains redox activity and structural stability, making it valuable for *in vitro* studies to dissect mechanisms of oxidative stress response, protein-protein interactions, and post-translational modifications. Researchers utilize recombinant NXN to explore its role in diseases linked to redox imbalance, including cancer, neurodegenerative disorders, and inflammatory conditions. For instance, its dysregulation has been implicated in tumor progression via Wnt signaling crosstalk and in neuroinflammation through NF-κB pathway modulation.
The development of recombinant NXN has accelerated drug discovery efforts, particularly in designing inhibitors or activators targeting redox-dependent pathways. Additionally, it serves as a tool to study thioredoxin family evolution and functional diversification. Despite progress, challenges remain in fully characterizing its context-dependent roles and tissue-specific interactions, highlighting the need for further structural and functional studies using recombinant protein-based models.
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