| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFPT2 |
| Uniprot No | O94808 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-682aa |
| 氨基酸序列 | CGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNHEVK ERHIQLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSVH YPGEAVATRR GSPLLIGVRS KYKLSTEQIP ILYRTCTLEN VKNICKTRMK RLDSSACLHA VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGVTNT VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL QNRRQEIIRG LRSLPELIKE VLSLEEKIHD LALELYTQRS LLVMGRGYNY ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GFPT2重组蛋白的3篇参考文献示例(文献为虚构，仅作格式参考)：
1. **文献名称**：*Expression and Purification of Recombinant GFPT2 in Escherichia coli for Functional Studies*
**作者**：Chen L, Wang Y, Zhang H.
**摘要**：本研究成功克隆并表达了人源GFPT2基因的重组蛋白，利用大肠杆菌表达系统进行高效纯化。通过酶活性测定证实重组蛋白具有催化果糖-6-磷酸转化为葡萄糖胺-6-磷酸的活性，为后续糖尿病相关代谢研究提供了工具。
2. **文献名称**：*Structural Insights into GFPT2 Regulation by Post-Translational Modifications*
**作者**：Kim S, Ito T, Garcia-Ruiz E.
**摘要**：通过X射线晶体学解析GFPT2重组蛋白的三维结构，发现其磷酸化位点对酶活性的调控机制，揭示了GFPT2在肿瘤细胞异常糖代谢中的潜在作用。
3. **文献名称**：*GFPT2 Knockdown and Recombinant Protein Rescue in Muscle Atrophy Models*
**作者**：Müller R, Santos A, Li J.
**摘要**：利用GFPT2重组蛋白在肌肉萎缩细胞模型中恢复谷氨酰胺代谢通路功能，证实GFPT2通过调控己糖胺生物合成途径影响肌肉细胞存活，为治疗提供新靶点。
(注：以上文献为模拟生成，实际研究需通过PubMed、Google Scholar等平台检索。)
**GFPT2 Recombinant Protein: Background and Significance**
Glutamine:fructose-6-phosphate amidotransferase 2 (GFPT2) is a key enzyme in the hexosamine biosynthesis pathway (HBP), which converts fructose-6-phosphate and glutamine into glucosamine-6-phosphate, a critical precursor for glycosylation. This post-translational modification is essential for protein folding, cellular signaling, and extracellular matrix interactions. GFPT2. one of two isoforms (GFPT1 and GFPT2), is predominantly expressed in tissues like skeletal muscle, pancreas, and the nervous system, where it regulates flux through the HBP in response to nutrient availability and metabolic stress.
Recombinant GFPT2 protein is produced using biotechnological methods, such as expression in bacterial or mammalian systems, followed by purification to ensure high enzymatic activity and stability. Its production enables researchers to study GFPT2’s structure-function relationships, kinetic properties, and regulatory mechanisms. Dysregulation of GFPT2 has been linked to metabolic disorders, including diabetes, as excessive HBP activity contributes to insulin resistance. In cancer, GFPT2 overexpression may promote tumor progression by enhancing glycosylation-driven processes like cell adhesion and metastasis. Additionally, GFPT2 mutations are associated with congenital myasthenic syndromes, highlighting its role in neuromuscular junction maintenance.
Research using recombinant GFPT2 protein also aids in developing inhibitors or modulators targeting the HBP, offering therapeutic potential for metabolic diseases, neurodegeneration, and cancer. By elucidating GFPT2’s role in cellular metabolism and disease, this recombinant tool advances our understanding of glycosylation’s broader implications in health and pathology.
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