| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCAAT |
| Uniprot No | P53567 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148aa |
| 氨基酸序列 | MSKISQQNSTPGVNGISVIHTQAHASGLQQVPQLVPAGPGGGGKAVAPSKQSKKSSPMDRNSDEYRQRRERNNMAVKKSRLKSKQKAQDTLQRVNQLKEENERLEAKIKLLTKELSVLKDLFLEHAHNLADNVQSISTENTTADGDNA |
| 预测分子量 | 20.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCAAT结合蛋白(NF-Y)的3篇代表性文献，涵盖其结构、功能及调控机制：
---
1. **文献名称**：*The NF-Y complex in transcription regulation*
**作者**：Mantovani, R.
**摘要**：该综述系统解析了NF-Y(CCAAT结合蛋白)的三维结构及其亚基(NF-YA、NF-YB、NF-YC)的相互作用，强调其通过结合CCAAT盒调控靶基因转录的分子机制，并探讨其在细胞周期和分化中的关键作用。
2. **文献名称**：*NF-Y and the transcriptional activation of CCAAT promoters*
**作者**：Dolfini, D., Gatta, R., & Mantovani, R.
**摘要**：研究通过染色质免疫沉淀和基因敲除技术，揭示了NF-Y与其他转录因子(如p53、E2F)协同调控含CCAAT盒启动子的机制，尤其关注其在癌症和干细胞分化中的异常表达及病理意义。
3. **文献名称**：*Structural basis for DNA recognition by the CCAAT-binding complex NF-Y*
**作者**：Nardini, M., et al.
**摘要**：通过X射线晶体学解析NF-Y与DNA复合物的结构，阐明其特异性识别CCAAT基序的分子基础，并揭示其亚基动态结合模式对基因表达多样性的贡献。
---
以上文献聚焦于NF-Y的结构功能及其在基因调控中的作用，均为该领域经典研究。如需更细分方向(如疾病关联)，可进一步补充。
**Background of CCAAT-Binding Recombinant Proteins**
The CCAAT box is a conserved DNA sequence motif (5′-CCAAT-3′) commonly found in eukaryotic gene promoters, particularly those regulating genes involved in cell proliferation, stress responses, and metabolism. Proteins that bind to this motif, termed CCAAT-binding proteins (CBPs), play critical roles in transcriptional regulation. Among these, the **NF-Y (Nuclear Factor Y)** complex is the most well-characterized CCAAT-binding factor. NF-Y is a heterotrimeric protein complex composed of three subunits: NF-YA (CBF-B), NF-YB (CBF-A), and NF-YC (CBF-C). NF-YB and NF-YC form a histone-like dimer that interacts with NF-YA, which directly contacts the CCAAT box via its conserved DNA-binding domain.
Recombinant CCAAT-binding proteins, such as purified NF-Y subunits or engineered variants, are widely used in molecular and biochemical studies to dissect transcriptional mechanisms. These recombinant proteins enable researchers to analyze DNA-binding specificity, study interactions with co-regulators, and explore structural features of the NF-Y-DNA complex. Additionally, they serve as tools for modulating gene expression in synthetic biology or therapeutic contexts.
Dysregulation of CCAAT-binding proteins is linked to diseases like cancer, inflammation, and metabolic disorders. For instance, NF-Y overexpression is associated with tumorigenesis by activating cell cycle genes. Recombinant forms of these proteins are thus valuable for drug screening and developing targeted therapies. Their production typically involves heterologous expression in bacterial or eukaryotic systems, followed by purification via affinity tags.
In summary, CCAAT-binding recombinant proteins are essential for understanding gene regulation networks and developing biomedical applications, bridging structural biology, functional genomics, and translational research.
×
