| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | ALT |
| Uniprot No | Q8QZR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-496aa |
| 氨基酸序列 | MASQRNDRIQASRNGLKGKVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYIERRDGGIPADPNNIFLSTGASDAIVTMLKLLVAGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDEERAWALDIAELRRALCQARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFEEGLFLMADEVYQDNVYAEGSQFHSFKKVLTEMGPPYATQQELASFHSVSKGYMGECGFRGGYVEVVNMDAEVQKQMAKLMSVRLCPPVPGQALMGMVVSPPTPSEPSFKQFQAERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQIQLPLKAVQRAQDLGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKLRVLLEKLRHFHAKFTHEYS |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组丙氨酸转氨酶(ALT)的示例参考文献(注：部分文献信息为示例性虚构，实际引用请查询学术数据库)：
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1. **文献名称**：High-level expression of recombinant human alanine aminotransferase in *Pichia pastoris*
**作者**：Chen L, et al.
**摘要**：研究利用毕赤酵母系统高效表达重组人ALT，优化发酵条件后获得高活性酶，为肝病诊断试剂盒的规模化生产提供技术支持。
2. **文献名称**：Purification and kinetic characterization of a novel recombinant ALT variant for metabolic studies
**作者**：Kim S, Park JH
**摘要**：通过亲和层析纯化重组ALT变体，分析其酶动力学参数，揭示其在氨基酸代谢中的潜在应用价值。
3. **文献名称**：ALT recombinant protein as a biomarker in drug-induced liver injury models
**作者**：Garcia R, et al.
**摘要**：评估重组ALT蛋白作为药物肝毒性模型的生物标志物，证实其在体外检测中与传统检测方法的高度一致性。
4. **文献名称**：Engineering thermostable ALT enzymes through directed evolution
**作者**：Müller T, Schmidt A
**摘要**：利用定向进化技术改造重组ALT蛋白，提升其热稳定性，拓展其在工业催化中的应用潜力。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索真实文献。若用户所指“ALT”为其他含义(如端粒相关蛋白)，需调整关键词重新查询。
**Background of ALT Recombinant Proteins**
Recombinant proteins, including alanine transaminase (ALT), are engineered through genetic modification to enable large-scale production in host systems like bacteria, yeast, or mammalian cells. ALT, a key enzyme in the alanine cycle, catalyzes the interconversion of alanine and pyruvate, playing a vital role in amino acid metabolism and gluconeogenesis. Traditionally, ALT enzymes for research or diagnostics were purified from animal tissues, but variability in quality, scalability, and ethical concerns drove the shift toward recombinant technology.
The development of recombinant ALT involves cloning the ALT gene into expression vectors, followed by transfection into host cells. Optimization of expression conditions ensures high yield and functional activity. Mammalian systems (e.g., HEK293 or CHO cells) are often preferred for producing post-translationally modified, human-like ALT, whereas bacterial systems offer cost-effective production but lack certain modifications.
Recombinant ALT is widely used in clinical diagnostics to assess liver function, as elevated serum ALT levels indicate hepatic damage. It also serves as a critical tool in drug toxicity studies, disease modeling (e.g., non-alcoholic fatty liver disease), and metabolic research. Advantages include batch-to-batch consistency, reduced animal dependency, and scalability. Challenges remain in achieving proper folding, post-translational modifications, and cost-efficient production for low-resource settings.
Recent advances in protein engineering, such as codon optimization and CRISPR-edited cell lines, aim to enhance ALT stability and activity. Additionally, recombinant ALT is being explored for therapeutic applications, including enzyme replacement therapies. As demand for precision biomolecules grows, recombinant ALT exemplifies the intersection of biotechnology, medicine, and industrial innovation.
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