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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BMP3 |
| Uniprot No | P12645 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 363-472aa |
| 氨基酸序列 | QWIEPRNCARRYLKVDFADIGWSEWIISPKSFDAYYCSGACQFPMPKSLKPSNHATIQSIVRAVGVVPGIPEPCCVPEKMSSLSILFFDENKNVVLKVYPNMTVESCACR |
| 预测分子量 | 13.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BMP3重组蛋白的3篇参考文献示例(文献标题和作者为虚构，仅用于示例格式)：
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1. **文献名称**: *Recombinant human BMP3 promotes osteogenic differentiation of mesenchymal stem cells via Smad-independent signaling*
**作者**: Smith A, et al.
**摘要**: 本研究通过表达纯化获得高活性人源BMP3重组蛋白，发现其通过激活MAPK通路而非经典Smad通路促进间充质干细胞的成骨分化，为骨再生治疗提供新靶点。
2. **文献名称**: *Expression and purification of bioactive BMP3 in E. coli: Optimization and functional characterization*
**作者**: Chen L, et al.
**摘要**: 报道了一种利用大肠杆菌表达系统高效生产BMP3重组蛋白的方法，通过体外细胞实验证实其抑制成纤维细胞增殖的活性，为大规模制备奠定基础。
3. **文献名称**: *BMP3 deficiency exacerbates high-fat diet-induced metabolic syndrome in mice*
**作者**: Wang Y, et al.
**摘要**: 利用BMP3重组蛋白干预实验，证明BMP3通过调节脂肪组织炎症和胰岛素敏感性改善代谢紊乱，提示其在代谢疾病中的潜在治疗价值。
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注：以上文献信息为模拟内容，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
**Background of BMP3 Recombinant Protein**
Bone Morphogenetic Protein 3 (BMP3), a member of the TGF-β superfamily, is a secreted signaling protein involved in regulating skeletal development, tissue homeostasis, and embryonic patterning. Unlike other BMPs known for promoting osteogenesis, BMP3 exhibits unique antagonistic roles, such as inhibiting osteoblast differentiation and bone formation by modulating BMP and activin signaling pathways. This dual regulatory function positions BMP3 as a critical mediator in balancing bone metabolism and repair processes.
Recombinant BMP3 is produced through genetic engineering, typically using expression systems like *E. coli*, mammalian cells, or insect cells, ensuring high purity and bioactivity. Its production enables detailed studies of BMP3’s structure-function relationships, receptor interactions (e.g., activin receptor-like kinases), and downstream signaling cascades. Researchers leverage recombinant BMP3 to explore its role in diseases like osteoporosis, osteoarthritis, and fibrodysplasia ossificans progressiva (FOP), where BMP signaling dysregulation is implicated.
In regenerative medicine, BMP3’s inhibitory effects are being harnessed to fine-tune bone regeneration strategies, particularly in scenarios requiring controlled osteogenesis. Additionally, its involvement in non-skeletal tissues, such as neural and cardiovascular systems, highlights broader therapeutic potential. Recent studies also investigate BMP3’s diagnostic value as a biomarker in certain cancers and metabolic disorders.
Despite progress, challenges remain in fully elucidating BMP3’s context-dependent actions and optimizing its clinical applications. Ongoing research aims to decode its complex signaling mechanisms and develop targeted therapies leveraging its unique regulatory properties.
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