| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CORO1A |
| Uniprot No | P31146 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-461aa |
| 氨基酸序列 | SRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFVALICEASGGGAFLVLPLGKTGRVDKNAPTVCGHTAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLMLPLREPVVTLEGHTKRVGIVAWHTTAQNVLLSAGCDNVIMVWDVGTGAAMLTLGPEVHPDTIYSVDWSRDGGLICTSCRDKRVRIIEPRKGTVVAEKDRPHEGTRPVRAVFVSEGKILTTGFSRMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERRCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPKSRELRVNRGLDTGRRRAAPEASGTPSSDAVSRLEEEMRKLQATVQELQKRLDRLEETVQAK |
| 预测分子量 | 57.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CORO1A重组蛋白的参考文献示例(文献标题、作者及摘要概括)：
1. **"Coronin 1A regulates neutrophil survival by modulating membrane recycling"**
*Authors: Shiow et al.*
摘要：该研究通过重组CORO1A蛋白实验，揭示了其在调控中性粒细胞膜循环中的作用，证明CORO1A通过影响囊泡运输通路抑制细胞凋亡，维持先天免疫细胞稳态。
2. **"Structural insights into the actin-binding function of human Coronin 1A"**
*Authors: Gatfield et al.*
摘要：利用重组CORO1A蛋白的晶体结构分析，阐明了其与F-actin结合的分子机制，发现特定结构域对细胞骨架重塑和免疫细胞迁移的关键调控作用。
3. **"Defective T cell development in mice lacking Coronin 1A"**
*Authors: Föger et al.*
摘要：通过构建CORO1A基因敲除小鼠模型，结合重组蛋白回补实验，证实CORO1A缺失导致T细胞成熟障碍，揭示其在胸腺细胞信号转导中的必要性。
注：以上为示例性内容，实际文献需通过数据库(如PubMed)按关键词检索。建议补充具体研究方向的限定词(如“疾病机制”“结构解析”)以提高检索精准度。
Coronin 1A (CORO1A) is a member of the coronin protein family, known for its role in regulating cytoskeletal dynamics, particularly actin filament organization. Primarily expressed in immune cells such as T lymphocytes and macrophages, CORO1A is crucial for cellular processes like migration, phagocytosis, and immune signaling. Structurally, it contains a conserved coronin domain with WD40 repeats, enabling interactions with actin and other signaling molecules. Its function extends beyond cytoskeletal remodeling, including modulation of calcium signaling and membrane trafficking.
CORO1A gained attention due to its association with severe combined immunodeficiency (SCID). Mutations in the CORO1A gene disrupt T-cell development and function, leading to immune dysregulation. This link highlights its essential role in maintaining immune homeostasis. Additionally, CORO1A interacts with pathogenic microbes; some intracellular pathogens exploit CORO1A to evade host defenses, making it a potential target for antimicrobial therapies.
Recombinant CORO1A protein is engineered for research and therapeutic applications. Produced via bacterial or eukaryotic expression systems, it retains functional domains for in vitro studies, such as investigating immune cell behavior, host-pathogen interactions, or actin-related mechanisms. Its recombinant form is also used to generate antibodies, study protein-protein interactions, or screen small-molecule inhibitors. Recent studies explore its diagnostic potential as a biomarker for immune disorders or infectious diseases. Despite progress, questions remain about its tissue-specific regulation and broader signaling networks, driving ongoing research into its multifaceted roles in health and disease.
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