| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAT |
| Uniprot No | P46402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-167aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSQAMFFKEIEELKGYFNASNPDVADGGS LFVDILKNWKEESDKTIIQSQIVSFYLKMFENLKDDDQRIQRSMDTIKED MLDKLLNTSSSKRDDFLKLIQIPVNDLQVQRKAINELFKVMNDLSPRSNL RKRKRSQNLFRGRRASK |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAT(氯霉素乙酰转移酶)重组蛋白的3篇模拟参考文献示例：
1. **《高效表达重组氯霉素乙酰转移酶在大肠杆菌中的优化》**
作者：Smith J, et al.
摘要：本研究通过密码子优化和诱导条件调控，在大肠杆菌中实现CAT蛋白的高效可溶性表达，酶活较传统方法提升3倍，为报告基因系统提供优化方案。
2. **《CAT融合蛋白在真核细胞转录调控研究中的应用》**
作者：Zhang L, Wang H.
摘要：构建CAT与多种启动子的融合载体，通过检测乙酰化活性定量评估哺乳动物细胞中特定信号通路的转录激活效率，验证其作为报告系统的稳定性。
3. **《重组CAT蛋白的晶体结构解析及底物结合机制》**
作者：Tanaka K, et al.
摘要：首次解析2.1Å分辨率的重组CAT三维结构，揭示氯霉素结合口袋的关键氨基酸残基，突变实验证实His195在催化中的核心作用，为抗生素耐药机制研究提供结构基础。
注：以上为模拟文献条目，实际引用时请检索真实数据库(如PubMed)获取权威信息。
**Background of CAT Recombinant Protein**
Chloramphenicol acetyltransferase (CAT) is a bacterial enzyme originally identified for its role in conferring resistance to the antibiotic chloramphenicol. It catalyzes the transfer of an acetyl group from acetyl-CoA to chloramphenicol, rendering the antibiotic inactive. Since its discovery, the *cat* gene encoding this enzyme has been widely repurposed in molecular biology as a reporter gene to study gene expression and promoter activity in both prokaryotic and eukaryotic systems.
Recombinant CAT protein is produced through genetic engineering, typically using *E. coli* or other expression hosts. Its small size (~25 kDa monomer, forming a homotrimer) and stability make it suitable for in vitro assays. In research, CAT activity is measured via colorimetric, radiometric, or fluorescent assays, often using acetyl-CoA derivatives. Historically, CAT was a cornerstone in studying transcriptional regulation due to its sensitivity and lack of endogenous background activity in mammalian cells.
While newer reporter systems (e.g., luciferase, GFP) have largely replaced CAT in high-throughput applications, it remains valuable in specific contexts, such as anaerobic studies (where oxygen-dependent reporters fail) or in validating gene delivery systems. Additionally, CAT’s role in antibiotic resistance mechanisms continues to inform studies on bacterial pathogenesis and horizontal gene transfer.
Overall, CAT recombinant protein exemplifies the adaptation of a bacterial defense molecule into a versatile tool for molecular biology, bridging foundational research and biotechnological applications.
×
